The influence of pulsed suction (PS) on flow separation and aerodynamic performance in a highly loaded compressor cascade is experimentally studied herein. The excitation frequency is investigated as it determines the effectiveness of PS in flow control. Low suction flowrates are examined to analyze the potential of PS in providing a satisfactory cascade performance. For comparison, the corresponding parameters of steady continuous suction (SCS) are studied as well. Oil-flow visualizations and steady and unsteady pressure data are used to characterize the control effects of SCS and PS. The experimental results validate the efficacy of PS in controlling flow separation, even at a reduced suction flowrate of 0.1%. It suppresses passage vortex (PV), improving aerodynamic performance. PS provides a better control effect than SCS at different excitation parameters, which can be attributed to twofold main reasons: first, at the same suction flowrate, PS has a larger suction momentum than SCS during the suction phase, resulting in a stronger suction force and having a more profound effect on the flow characteristics; and second, owing to the introduction of pulsed excitation to the suction, PS creates additional vortex structures that energize the boundary layer by transporting high momentum freestream fluid near the wall. PS is also effective at a higher incidence angle, but its control effect is reduced.