The transient mixed convection boundary layer analysis of incompressible flow over an isothermal vertical cylinder is embedded in a saturated porous medium in the vicinity for Gyrotactic microorganism effects. The mathematical model used for the bioconvective nanofluid incorporates the effects of Brownian motion, thermophoresis, and gyrotactic microorganisms. Moreover, the resulting governing nonsimilarity equations are changed into partial differential equations and solved numerically. The results are explained graphically for various physical parameters. It is determined that bioconvection parameter boosts the heat transfer rates and the thickness of the motile microorganism reduces the mass transfer rates. Expanding bioconvection Lewis number leads to decrease in heat transfer rates and the density of the motile microorganism, whereas the mass transfer rates decelerate the flow field. The investigation is pertinent to the nanobiopolymer manufacturing processes.