Abstract

Fluorescent tracer dyes are popularly used in agricultural spray application studies to evaluate on-target canopy deposition and off-target drift, and the methods/instruments used for evaluating dye deposit must assure reliability of the data generated. A laboratory setup was configured to adapt a submersible fluorometer for controlled and reliable measurement of pyranine dye fluorescence in liquid samples. The system setup was blanked using a clean sample of de-ionized water and calibrated using standard dye solutions of known concentration (100 to 1,000,000 parts per trillion [ppt]) made from two dyes of different levels of purity (pyranine 98 % and pyranine 85 %). Pyranine 85 % fluorescence readings were consistently lower with reduction factor averaging ∼0.62 times that of pyranine 98 % within a range from 500 to 1,000,000 ppt. The results were verified using a standard laboratory fluorometer. Spray deposition assessment of string, plastic card, cotton ribbon, and artificial foliage samplers was accomplished using the configured fluorometer system setup. The results showed no significant difference among dye purity levels (P = 0.430), no statistically significant interaction between dye purity and concentration (P = 0.484), and no statistically significant interaction between dye purity and sampler type (P = 0.173). Consequently, the configured setup can produce measurements with similar quality as the standard laboratory fluorometer and both dyes tested may be equally used for spray deposition and drift assessments, or for similar applications.

References

1.
Zhu
H.
,
Derksen
R. C.
,
Krause
C. R.
,
Fox
R. D.
,
Brazee
R. D.
, and
Ozkan
H. E.
, “
Effect of Solution pH Conditions on Fluorescence of Spray Deposition Tracers
,”
Applied Engineering in Agriculture
21
, no. 
3
(
2005
):
325
329
, https://doi.org/10.13031/2013.18447
2.
Derksen
R. C.
,
Zhu
H.
,
Fox
R. D.
,
Brazee
R. D.
, and
Krause
C. R.
, “
Coverage and Drift Produced by Air Induction and Conventional Hydraulic Nozzles Used for Orchard Applications
,”
Transactions of the ASABE
50
, no. 
5
(
2007
):
1493
1501
, https://doi.org/10.13031/2013.23941
3.
Khot
L. R.
,
Ehsani
R.
,
Albrigo
G.
,
Larbi
P. A.
,
Landers
A.
,
Campoy
J.
, and
Wellington
C.
, “
Air-Assisted Sprayer Adapted for Precision Horticulture: Spray Patterns and Deposition Assessments in Small-Sized Citrus Canopies
,”
Biosystems Engineering
113
, no. 
1
(September
2012
):
76
85
, https://doi.org/10.1016/j.biosystemseng.2012.06.008
4.
Larbi
P. A.
and
Salyani
M.
, “
Model to Predict Spray Deposition in Citrus Airblast Sprayer Applications—Part 1: Spray Dispersion
,”
Transactions of the ASABE
55
, no. 
1
(
2011
):
29
39
, https://doi.org/10.13031/2013.41245
5.
Pergher
G.
, “
Recovery Rate of Tracer Dyes Used for Spray Deposit Assessment
,”
Transactions of the ASABE
44
, no. 
4
(
2001
):
787
794
, https://doi.org/10.13031/2013.6240
6.
Field
M. S.
,
Wilhelm
R. G.
,
Quinlan
J. F.
, and
Aley
T. J.
, “
An Assessment of the Potential Adverse Properties of Fluorescent Tracer Dyes Used for Groundwater Tracing
,”
Environmental Monitoring and Assessment
38
, no. 
1
(October
1995
):
75
96
, https://doi.org/10.1007/BF00547128
7.
Lakowicz
J. R.
,
Principles of Fluorescence Spectroscopy
, 3rd ed. (
New York
:
Springer Science+Business Media
,
2006
), https://doi.org/10.1007/978-0-387-46312-4
8.
Legenzov
E. A.
,
Dirda
N. D. A.
,
Hagen
B. M.
, and
Kao
J. P. Y.
, “
Synthesis and Characterization of 8-O-Carboxymethylpyranine (CM-Pyranine) as a Bright, Violet-Emitting, Fluid-Phase
,”
PLoS ONE
10
, no. 
7
(July
2015
): e0133518, https://doi.org/10.1371/journal.pone.0133518
9.
Levitus
M.
and
Ranjit
S.
, “
Cyanine Dyes in Biophysical Research: The Photophysics of Polymethine Fluorescent Dyes in Biomolecular Environments
,”
Quarterly Reviews of Biophysics
44
, no. 
1
(
2011
):
123
151
, https://doi.org/10.1017/S0033583510000247
10.
Klymchenko
A. S.
, “
Solvatochromic and Fluorogenic Dyes as Environment-Sensitive Probes: Design and Biological Applications
,”
Accounts of Chemical Research
50
, no. 
2
(January
2017
):
366
375
, https://doi.org/10.1021/acs.accounts.6b00517
11.
Karakolisa
E. G.
,
Nguyena
B.
,
You
J. B.
,
Rochmanb
C. M.
, and
Sintona
D.
, “
Fluorescent Dyes for Visualizing Microplastic Particles and Fibers in Laboratory-Based Studies
,”
Environmental Science & Technology Letters
6
, no. 
6
(May
2019
):
334
340
, https://doi.org/10.1021/acs.estlett.9b00241
12.
Salyani
M.
,
Farooq
M.
, and
Sweeb
R. D.
, “
Spray Deposition and Mass Balance in Citrus Orchard Applications
,”
Transactions of the ASABE
50
, no. 
6
(
2007
):
1963
1969
, https://doi.org/10.13031/2013.24092
13.
Larbi
P. A.
and
Salyani
M.
, “
CitrusSprayEx: An Expert System for Planning Citrus Spray Applications
,”
Journal of Computers and Electronics in Agriculture
87
(September
2012
):
85
93
, https://doi.org/10.1016/j.compag.2012.05.005
14.
Salyani
M.
,
Zhu
H.
,
Sweeb
R. D.
, and
Pai
N.
, “
Assessment of Spray Distribution with Water-Sensitive Paper
,”
Agricultural Engineering International: CIGR Journal
15
, no. 
2
(July
2013
):
101
111
.
15.
Farooq
M.
and
Salyani
M.
, “
Spray Penetration into the Citrus Tree Canopy from Two Air–Carrier Sprayers
,”
Transactions of the ASABE
45
, no. 
5
(
2002
):
1287
1293
, https://doi.org/10.13031/2013.11057
16.
Salyani
M.
, “
Optimization of Deposition Efficiency for Airblast Sprayers
,”
Transactions of the ASAE
43
, no. 
2
(
2000
):
247
253
, https://doi.org/10.13031/2013.2699
17.
Nairn
J. J.
and
Forster
W. A.
, “
Photostability of Pyranine and Suitability as a Spray Drift Tracer
,”
New Zealand Plant Protection
68
(January
2015
):
32
37
, https://doi.org/10.30843/nzpp.2015.68.5795
18.
Larbi
P. A.
, “
Development of a Model to Predict Spray Deposition in Air-Carrier Sprayer Applications
” (PhD diss.,
University of Florida
,
2011
).
19.
Larbi
P. A.
and
Salyani
M.
, “
Model to Predict Spray Deposition in Citrus Airblast Sprayer Applications—Part 2: Spray Deposition
,”
Transactions of the ASABE
55
, no. 
1
(
2012
):
41
48
, https://doi.org/10.13031/2013.41246
20.
Hewitt
A.
,
Dorr
G.
,
Hughes
P.
, and
Axford
T.
,
Natural and Artificial Barriers for Spray Drift Exposure Mitigation in South Australia
(
Gatton, Australia
:
The Centre for Pesticide Application and Safety, The University of Queensland
,
2009
).
21.
Khot
L. R.
,
Salyani
M.
, and
Sweeb
R. D.
, “
Solar and Storage Degradations of Oil- and Water-Soluble Fluorescent Dyes
,”
Applied Engineering in Agriculture
27
, no. 
2
(
2011
):
211
216
, https://doi.org/10.13031/2013.36489
22.
Rathnayake
A. P.
,
Khot
L. R.
,
Hoheisel
G. A.
,
Thistle
H. W.
,
Teske
M. E.
, and
Willett
M. J.
, “
Downwind Spray Drift Assessment for Airblast Sprayer Applications in a Modern Apple Orchard System
,”
Transactions of the ASABE
64
, no. 
2
(
2021
):
601
613
, https://doi.org/10.13031/trans.14324
23.
Turner Designs
Databank Manual
(
San Jose, CA
:
Turner Designs, Inc.
,
2018
).
24.
Turner Designs
Cyclops Submersible Sensors: User’s Manual, Revision 3.0 ed.
(
San Jose, CA
:
Turner Designs, Inc.
,
2020
).
25.
Salyani
M.
, “
Methodologies for Assessment of Spray Deposition in Orchard Applications
” (paper presentation, 2000 ASAE Annual International Meeting, Milwaukee, WI, July 9–12,
2000
).
27.
Salyani
M.
,
Sweeb
R. D.
, and
Farooq
M.
, “
Spray Capture Efficiencies of Ribbon and String Targets Used in Orchard Applications
,” in
2006 ASAE Annual Meeting
(
American Society of Agricultural and Biological EngineersSt. Joseph, MI
:
American Society of Agricultural and Biological Engineers
,
2006
): 061127, https://doi.org/10.13031/2013.20629
28.
Vong
C. N.
and
Larbi
P. A.
, “
Development and Prototype Testing of an Agricultural Nozzle Clog Detection Device
,”
Transactions of the ASABE
64
, no. 
1
(
2021
):
49
61
, https://doi.org/10.13031/trans.13519
29.
UCLA Statistics “
FAQ: What Is the Coefficient of Variation?
” UCLA: Statistical Consulting Group, http://web.archive.org/web/20211216173206/https://stats.idre.ucla.edu/other/mult-pkg/faq/general/faq-what-is-the-coefficient-of-variation
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