Abstract

Adult Daphnia magna were exposed to 14C-labeled phenol and tissues analyzed for 14C uptake by three methods: (1) tissue solubilizer, (2) tissue solubilizer plus sonication, and (3) sonication alone. Analysis by liquid scintillation counting revealed that measurements of total activity among treatments were not significantly different (α ≤ 0.10) at two count levels. Sonicated samples showed less variation than tissue samples that were solubilized.

References

1.
Southworth
,
G. R.
,
Beauchamp
,
J. J.
, and
Schmeider
,
P. K.
, “
Bioaccumulation Potential and Acute Toxicity of Synthetic Fuels Effluents in Freshwater Biota: Azaarenes
,”
Environmental Science and Technology
 0013-936X, Vol.
12
, No.
9
,
09
1978
, pp.
1062
-
1066
.
2.
Herbes
,
S. E.
and
Risi
,
G. F.
, “
Metabolic Alteration and Excretion of Anthracene by Daphnia pulex
,”
Bulletin of Environmental Contamination and Toxicology
 0007-4861, Vol.
19
, No.
2
,
1978
, pp.
147
-
155
.
3.
Leversee
,
G. J.
,
Giesy
,
J. P.
, Jr.
,
Landrum
,
P. F.
,
Bartell
,
S.
,
Gould
,
S.
,
Bruns
,
M.
,
Spacie
,
A.
,
Bowling
,
J.
,
Haddock
,
J.
, and
Fannin
,
T.
, “
Disposition of Benzo (a) Pyrene in Aquatic Systems Components: Periphyton, Chironomids, Daphnia, Fish
,” in
Polynuclear Aromatic Hydrocarbons
,
Battelle Laboratories
,
Columbus, OH
,
1980
.
4.
Wang
,
C. H.
,
Willis
,
D. L.
, and
Loveland
,
W. D.
,
Radiotracer Methodology in the Biological, Environmental, and Physical Sciences
.
Prentice-Hall, Inc.
,
Englewood Cliffs, NJ
,
1975
.
5.
Winer
,
B. J.
,
Statistical Principles in Experimental Design
, 2nd ed.,
McGraw Hill Book Co.
,
New York
,
1971
.
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