Separation of cancer cells from the other biological cells is important for clinical cancer diagnosis and cancer treatment. In this presentation, we use conventional dielectrophoresis (c-DEP) in a microfluidic chip to manipulate and collect colorectal cancer HCT116 cell. It is noticed that at particular AC frequency band, the HCT116 cell are deflected to a side channel from a main channel clearly after the electric activation. This motion caused by negative DEP can be used to separate the cancer cell from others. In this manuscript, we report the chip design, flow condition, the DEP spectrum of the cancer cell, and the separation and collection efficiency as well.