We report on the enhancement of naturally-occurred self ordering of nanopores in anodic aluminium oxide (AAO) membrane by performing three-step anodic oxidation process. Naturally-occurred self ordering of nanopores in anodic aluminium oxide (AAO) membrane has brought it into the applications of template for fabrication of nanoscale materials. Three-step anodic oxidation method was used to achieve self-ordering of nanopores. The effect of duration of first and second steps on the ordering of nanopores was investigated. The current-time curves recorded during anodization elucidate an almost same behavior for all three steps. Scanning electron micrographs (SEM) show hexagonally arranged 45 nm pores in a manner which contribute into the formation of highly ordered areas, called domains. Larger ones are clearly observed over the surface, for samples with longer first and second anodization steps.