Eurofer97 is one of leading candidates of reduced activation ferritic martensitic (RAFM) steels for first wall structural materials of early demonstration fusion power plants. During fusion plant operation, high neutron irradiation damage on first wall materials can cause irradiation embrittlement and reduce the fracture toughness of RAFM steels. Therefore, it is critical to select proper testing techniques to characterize the fracture toughness of RAFM steels with high fidelity. In this manuscript, we present the feasibility study of using pre-cracked miniature multi-notch bend bar specimens (M4CVN) with a dimension of 45mm (length) × 3.3mm (width) × 1.65mm (thickness) to characterize the transition fracture toughness of Eurofer97 steel based on the ASTM E1921 Master Curve method. The testing yielded a provisional Master Curve reference temperature T oQ of −89°C of unirradiated Eurofer97 steel heat J362A in the normalized and tempered condition. The results are within the normal scatter range of Master Curve reference temperature T 0 for Eurofer97 steel, indicating suitability of applying M4CVN specimens for characterizing the transition fracture toughness of Eurofer97 steel.