Pressure loss coefficients are generally required by subchannel and system thermalhydraulics codes. These coefficients are not readily available for small modular reactors (SMRs) featuring nonconventional designs and novel coolants. In this study, the pressure loss coefficients were obtained using three-dimensional (3D) computational fluid dynamics (CFD) modeling for an advanced water-cooled reactor. A representative light water fuel assembly used in the Organization for Economic Co-operation and Development (OECD)/National Research Council Canada (NRC) pressurized water reactor subchannel and bundle tests (PSBT) benchmark was selected for CFD modeling and simulation under various working conditions. The fuel assembly includes three types of pressurized water reactor (PWR) spacer grids: simple grid (SG), nonmixing vane grid (NMVG), and mixing vane grid (MVG). Turbulent flow through subchannels of both nonheated and heated rod bundles was simulated to predict recoverable and nonrecoverable pressure distribution along the length of the bundle. It was observed that vortices were generated at the tips of spacer grids, affecting the cross-flow in subchannels significantly. The estimated pressure loss coefficients were found to be influenced by the flow conditions (Reynolds number or the upstream flow history) and spacer grid configuration. Pressure loss coefficient values ranged from 1.14 to 1.80, depending on the spacer grid type, design, and flow conditions. The CFD method used in this study was demonstrated to have the potential to generate input parameters required for the subchannel analysis and optimization of fuel assembly designs and serve as a surrogate for empirical correlations.