Here, we report a threshold limit value (TLV) of on-chip cytotoxicity of Nepali Chiya, the Nepali traditional black tea. To demonstrate our proof-of-concept validation, we used the active sealing chip with serial dilution that can directly perform on-chip cytotoxicity testing onto the cells cultured in a petri dish. In our experiments, the TLV for mortality on HeLa cells was observed as 400 μg/ml for Nepali Chiya extract. We believe this approach would be a rapid and simple method for on-chip TLV screening of potability of tea extract at the laboratory level, and furthermore as a new potential drug supplement in pharmaceutical industries.

References

References
1.
Suhardiman
,
D.
,
Clement
,
F.
, and
Bharati
,
L.
,
2015
, “
Integrated Water Resources Management in Nepal: Key Stakeholders' Perceptions and Lessons Learned
,”
Int. J. Water Resour. Dev.
,
31
(
2
), pp.
284
300
.
2.
Menger
,
L. M.
,
Kaufman
,
M. R.
,
Harman
,
J. J.
,
Tsang
,
S. W.
, and
Shrestha
,
D. K.
,
2015
, “
Unveiling the Silence: Women's Sexual Health and Experiences in Nepal
,”
Cult. Health Sexuality
,
17
(
3
), pp.
359
373
.
3.
Walboomers
,
J. M.
,
Jacobs
,
M. V.
,
Manos
,
M. M.
,
Bosch
,
F. X.
,
Kummer
,
J. A.
,
Shah
,
K. V.
,
Snijders
,
P. J.
,
Peto
,
J.
,
Meijer
,
C. J.
, and
Munoz
,
N.
,
1999
, “
Human Papillomavirus is a Necessary Cause of Invasive Cervical Cancer Worldwide
,”
J. Pathol.
,
189
(
1
), pp.
12
19
.
4.
Ann
,
C. W.
, and
Yeo
,
S. G.
,
1996
, “
The Nitrite-Scavenging Effects by Component of Oolong and Black Tea Extracts
,”
Korean J. Life Sci.
,
6
(
2
), pp.
104
110
.
5.
Lee
,
B. R.
,
Park
,
J. Y.
, and
Park
,
P. S.
,
2011
, “
Effect of Green Tea Extract on Cisplatin- or Doxorubicin-Induced Cytotoxicity in Human Lung Cancer Cell Lines
,”
J. Korean Soc. Food Sci. Nutr.
,
40
(
5
), pp.
619
624
.
6.
Lee
,
J. S.
,
Oh
,
S. Y.
,
Seo
,
S.
,
Kim
,
T.
, and
Ma
,
J.
,
2011
, “
Protective Effect of Socheongryong-Tang on Hydrogen Peroxide-Induced Hepatotoxicity
,”
Korean J. Herbology
,
26
(
4
), pp.
133
137
.
7.
Kim
,
H. Y.
,
2013
, “
Effects of Water Extracts of Black Tea on Hepatic Functional Improvement and Anti-Fibrosis in Rats
,”
J. East Asian Soc. Diet. Life
,
23
(
1
), pp.
44
52
.
8.
Yin
,
B. S.
,
Li
,
M.
,
Liu
,
B. M.
,
Wang
,
S. Y.
, and
Zhang
,
W. G.
,
2015
, “
An Integrated Microfluidic Device for Screening the Effective Concentration of Locally Applied Tacrolimus for Peripheral Nerve Regeneration
,”
Exp. Ther. Med.
,
9
(
1
), pp.
154
158
.
9.
Lee
,
W. G.
,
Kim
,
Y. G.
,
Chung
,
B. G.
,
Demirci
,
U.
, and
Khademhosseini
,
A.
,
2010
, “
Nano/Microfluidics for Diagnosis of Infectious Diseases in Developing Countries
,”
Adv. Drug Delivery Rev.
,
62
(
4–5
), pp.
449
457
.
10.
Bang
,
H.
,
Lee
,
W. G.
,
Yun
,
H.
,
Chung
,
C.
,
Chang
,
J. K.
, and
Han
,
D. C.
,
2008
, “
A Directly Stackable Microsystem Onto the Cultured Cells for Cytotoxicity Tests
,”
Microsyst. Technol. Micro Nanosyst. Inf. Storage Process. Syst.
,
14
(
6
), pp.
719
724
.
11.
Yun
,
H.
,
Kim
,
K.
, and
Lee
,
W. G.
,
2013
, “
Cell Manipulation in Microfluidics
,”
Biofabrication
,
5
(
2
), pp. 1–14.
12.
Bang
,
H.
,
Lee
,
W. G.
,
Park
,
J.
,
Yun
,
H.
,
Lee
,
J.
,
Chung
,
S.
,
Cho
,
K.
,
Chung
,
C.
,
Han
,
D. C.
, and
Chang
,
J. K.
,
2006
, “
Active Sealing for Soft Polymer Microchips: Method and Practical Applications
,”
J. Micromech. Microeng.
,
16
(
4
), pp.
708
714
.
13.
Tian
,
X. L.
,
Wang
,
S. Y.
,
Zhang
,
Z.
, and
Lv
,
D. C.
,
2012
, “
Rat Bone Marrow-Derived Schwann-Like Cells Differentiated by the Optimal Inducers Combination on Microfluidic Chip and Their Functional Performance
,”
Plos One
,
7
(
8
), pp.
1
11
.
14.
Selimovic
,
S.
,
Sim
,
W. Y.
,
Kirn
,
S. B.
,
Jang
,
Y. H.
,
Lee
,
W. G.
,
Khabiry
,
M.
,
Bae
,
H.
,
Jambovane
,
S.
,
Hong
,
J. W.
, and
Khademhosseini
,
A.
,
2011
, “
Generating Nonlinear Concentration Gradients in Microfluidic Devices for Cell Studies
,”
Anal. Chem.
,
83
(
6
), pp.
2020
2028
.
You do not currently have access to this content.