Fabrication of cellular spheroids is critical for creating functional tissue units and investigating the mechanism of tumorigenesis, development, and intercellular and cell–matrix interactions in vitro. Herein, we developed a novel, simple, and facile method for cell spheroid fabrication by using polyacrylamide/gelatin methacrylate (PA/GelMA) hydrogel composites. Arrays of Michigan Cancer Foundation-7 (MCF-7) breast cancer cell spheroids can be easily formed by tuning the GelMA composition. The shape and size of cell spheroids can be also well controlled by regulating cell seeding density and culturing time. All these results suggested that this simple and facile platform can serve as a useful tool to generate three-dimensional (3D) cell spheroids and can be integrated within high-throughput drug screening platforms, which will be of great help in engineering functional tissue models and regenerative medicines.
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August 2015
Technical Briefs
Polyacrylamide/GelMA Hydrogel Templates for Breast Cancer Cell Spheroids Fabrication
Yuhui Li,
Yuhui Li
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Bioinspired Engineering and Biomechanics
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: yuhuili1988@gmail.com
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: yuhuili1988@gmail.com
Search for other works by this author on:
Xiang Li,
Xiang Li
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Bioinspired Engineering and Biomechanics
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: lixiang199109@gmail.com
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: lixiang199109@gmail.com
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Lu Zheng,
Lu Zheng
Key Laboratory of Microgravity (National
Microgravity Laboratory),
Center of Biomechanics and Bioengineering,
Institute of Mechanics,
Chinese Academy of Sciences,
Beijing 100190, China;
Microgravity Laboratory),
Center of Biomechanics and Bioengineering,
Institute of Mechanics,
Chinese Academy of Sciences,
Beijing 100190, China;
Beijing Key Laboratory of Engineered
Construction and Mechanobiology,
Institute of Mechanics,
Chinese Academy of Sciences,
Beijing 100190, China
e-mail: taswzzx@163.com
Construction and Mechanobiology,
Institute of Mechanics,
Chinese Academy of Sciences,
Beijing 100190, China
e-mail: taswzzx@163.com
Search for other works by this author on:
Lin Wang,
Lin Wang
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Bioinspired Engineering and Biomechanics
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: wanglin0527@126.com
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: wanglin0527@126.com
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Xiaohui Zhang,
Xiaohui Zhang
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Bioinspired Engineering and Biomechanics
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: xiaohuizhang@mail.xjtu.edu.cn
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: xiaohuizhang@mail.xjtu.edu.cn
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Feng Xu
Feng Xu
Mem. ASME
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Bioinspired Engineering and Biomechanics
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: fengxu@mail.xjtu.edu.cn
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: fengxu@mail.xjtu.edu.cn
Search for other works by this author on:
Yuhui Li
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Bioinspired Engineering and Biomechanics
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: yuhuili1988@gmail.com
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: yuhuili1988@gmail.com
Xiang Li
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Bioinspired Engineering and Biomechanics
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: lixiang199109@gmail.com
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: lixiang199109@gmail.com
Lu Zheng
Key Laboratory of Microgravity (National
Microgravity Laboratory),
Center of Biomechanics and Bioengineering,
Institute of Mechanics,
Chinese Academy of Sciences,
Beijing 100190, China;
Microgravity Laboratory),
Center of Biomechanics and Bioengineering,
Institute of Mechanics,
Chinese Academy of Sciences,
Beijing 100190, China;
Beijing Key Laboratory of Engineered
Construction and Mechanobiology,
Institute of Mechanics,
Chinese Academy of Sciences,
Beijing 100190, China
e-mail: taswzzx@163.com
Construction and Mechanobiology,
Institute of Mechanics,
Chinese Academy of Sciences,
Beijing 100190, China
e-mail: taswzzx@163.com
Lin Wang
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Bioinspired Engineering and Biomechanics
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: wanglin0527@126.com
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: wanglin0527@126.com
Xiaohui Zhang
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Bioinspired Engineering and Biomechanics
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: xiaohuizhang@mail.xjtu.edu.cn
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: xiaohuizhang@mail.xjtu.edu.cn
Feng Xu
Mem. ASME
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
The Key Laboratory of Biomedical Information
Engineering of Ministry of Education,
School of Life Science and Technology,
Xi'an Jiaotong University,
Xi'an 710049, China;
Bioinspired Engineering and Biomechanics
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: fengxu@mail.xjtu.edu.cn
Center (BEBC),
Xi'an Jiaotong University,
Xi'an 710049, China
e-mail: fengxu@mail.xjtu.edu.cn
1Corresponding author.
Manuscript received October 8, 2015; final manuscript received October 15, 2015; published online March 8, 2016. Assoc. Editor: Abraham Quan Wang.
J. Nanotechnol. Eng. Med. Aug 2015, 6(3): 034501 (5 pages)
Published Online: March 8, 2016
Article history
Received:
October 8, 2015
Revised:
October 15, 2015
Accepted:
October 19, 2015
Citation
Li, Y., Li, X., Zheng, L., Wang, L., Zhang, X., and Xu, F. (March 8, 2016). "Polyacrylamide/GelMA Hydrogel Templates for Breast Cancer Cell Spheroids Fabrication." ASME. J. Nanotechnol. Eng. Med. August 2015; 6(3): 034501. https://doi.org/10.1115/1.4031898
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