The effect of nanoparticles on subzero biotransport phenomena of living cells is very rare in the literature, although the information is of great importance for the application of nanotechnology in the field of cryobiology. In this study, subzero water transport phenomena in freezing HeLa cells in 1 × phosphate buffered saline (PBS) containing 0%, 0.05%, and 0.1% (w/w) hydroxyapatite (HA) nanoparticles with and without pre-incubation at 37 °C was quantitatively investigated. The results reveal that the presence of HA nanoparticles slightly facilitates the subzero water transport of HeLa cells.

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