The mixing efficiency of red blood cells and magnetic microspheres with red blood cell tagging ability is investigated using color particle image velocimetry. A hybrid separation system that utilizes centrifugation and magnetic separation is described. Magnetic microspheres are utilized to isolate the red cells from the buffy coat on a continuous basis. The effectiveness of red blood cell separation from the buffy coat in the hybrid system was improved over conventional centrifugal separation methods.

1.
Safarik
,
I.
, and
Safarikova
,
M.
, 1999, “
Use of Magnetic Techniques for the Isolation of Cells
,”
J. Chromatogr., B: Biomed. Appl.
0378-4347,
722
, pp.
33
53
.
2.
Ramadan
,
Q.
,
Samper
,
V.
,
Poenar
,
D. P.
, and
Yu
,
C.
, 2006, “
An Integrated Microfluidic Platform for Magnetic Microbeads Separation and Confinement
,”
Biosens. Bioelectron.
,
21
, pp.
1693
1702
. 0956-5663
3.
Lara
,
O.
,
Tong
,
X.
,
Zborowski
,
M.
, and
Chalmers
,
J. J.
, 2004, “
Enrichment of Rare Cancer Cells Through Depletion of Normal Cells Using Density and Flow-Through Immunomagnetic Cell Separation
,”
Exp. Hematol.
,
32
, pp.
891
904
. 0301-472X
4.
Haik
,
Y.
,
Pai
,
V.
, and
Chen
,
C. J.
, 1999, “
Development of Magnetic Device for Cell Separation
,”
J. Magn. Magn. Mater.
0304-8853,
194
, pp.
254
261
.
5.
Melville
,
D. F.
,
Paul
,
F.
, and
Roath
,
S.
, 1975, “
Direct Magnetic Separation of Red Cells From Whole Blood
,”
Nature (London)
0028-0836,
255
, p.
706
.
6.
Rous
,
P.
, and
Beard
,
J. W.
, 1934, “
Selection With the Magnet and Cultivation of Reticuloendothelial Cells (Kupffer Cells)
,”
J. Exp. Med.
0022-1007,
59
, pp.
577
591
.
7.
Owen
,
C. S.
, and
Lindsay
,
J. G.
, 1983, “
Ferritin as a Label for High Gradient Magnetic Separation
,”
Indian J. Biochem. Biophys.
0301-1208,
42
, pp.
145
150
.
8.
Graham
,
M.
, and
Selvin
,
P.
, 1982, “
Separation of Lanthanide Binding Cells
,”
IEEE Trans. Magn.
0018-9464,
18
(
6
), pp.
1523
1525
.
9.
Nakamura
,
T.
,
Konno
,
K.
,
Morone
,
T.
,
Tsuya
,
N.
, and
Hatano
,
M.
, 1971, “
Magneto Medicine: Biological Aspects of Ferromagnetic Fine Particles
,”
J. Appl. Phys.
0021-8979,
42
(
4
), pp.
1320
1324
.
10.
Hildegard
,
T. G.
,
Beatrix
,
V.
,
Werner
,
R.
,
Bernd
,
G.
,
Carlos
,
G. P.
,
Peter
,
K.
,
Jean
,
K.
,
Herbert
,
H.
,
Marina
,
C.
, and
Robert
,
K. M.
, 1998, “
Successful Use of Extracorporeal Photochemotherapy in the Treatment of Severe Acute and Chronic Graft-Host Disease
,”
Blood
,
92
, pp.
3098
3104
. 0006-4971
11.
Chatterjee
,
J.
,
Haik
,
Y.
, and
Chen
,
C. J.
, 2001, “
Modification and Characterization of Polystyrene-Based Magnetic Microspheres and Comparison With Albumin-Based Magnetic Microspheres
,”
J. Magn. Magn. Mater.
0304-8853,
225
, pp.
21
29
.
12.
Chattarjee
,
J.
,
Haik
,
Y.
, and
Chen
,
C.-J.
, 2001, “
Synthesis and Characterization of Heat Stabilized Albumin Magnetic Microspheres
,”
Colloid Polym. Sci.
,
279
, pp.
1073
1081
. 0303-402X
13.
Haik
,
Y.
,
Chen
,
C.-J.
,
Chatterjee
,
J.
, and
Kanuri
,
S.
, 2000, “
The Use of Biotinylated Lectin for Separating Red Cells From Whole Blood
,”
Biomol. Eng.
,
16
, p.
179
. 1389-0344
14.
Yu
,
S. C. M.
, and
Yip
,
T. H.
, 1997, “
Experimental Investigation of Two-Stream Mixing Flows With Streamwise and Normal Vorticity
,”
Int. J. Heat Fluid Flow
0142-727X,
18
, pp.
253
261
.
15.
Waitz
,
I. A.
,
Qiu
,
Y. J.
,
Manning
,
T. A.
,
Fung
,
A. K. S.
,
Elliot
,
J. K.
,
Kerwin
,
J. M.
,
Krasnodebski
,
J. K.
,
Sullivan
,
M. N. O.
,
Tew
,
D. E.
,
Greitzer
,
E. M.
,
Marble
,
F. E.
,
Tan
,
C. S.
, and
Tillman
,
T. G.
, 1997, “
Enhanced Mixing With Streamwise Vorticity
,”
Prog. Aerosp. Sci.
0376-0421,
33
, pp.
323
351
.
16.
Jaw
,
S. Y.
,
Haik
,
Y.
, and
Chen
,
C.-J.
, 2000, “
Development of Color Particle Image Velocimetry and Its Applications in Rotating Flow
,
Ninth International Symposium on Flow Visualization
, Edinburgh, Scotland, UK, Aug. 22–25, pp.
152
.
17.
Jandl
,
J. H.
, 1987,
Blood: A Textbook of Hematology
,
Little Brown
,
Boston
, pp.
264
265
.
18.
Blackshear
,
P. L.
, Jr.
, 1966, “
Shear, Wall Interaction and Hemolysis
,”
Trans. Am. Soc. Artif. Intern. Organs
,
12
, pp.
113
120
. 0066-0078
19.
Nevaril
,
C. G.
,
Lynch
,
E. C.
,
Alfrey
,
C. P.
, and
Hellums
,
J. D.
, 1968, “
Erythrocyte Damage and Destruction Induced by Shearing Stress
,”
J. Lab. Clin. Med.
,
71
, pp.
784
790
. 0022-2143
You do not currently have access to this content.