We report the use of a high resolution magnetic resonance (MR) imaging technique to monitor the development and maturation of tissue-printed constructs in vivo. Layer-by-layer inkjet printing technology was used to fabricate three different tissue constructs on alginate∕collagen gels: bovine aortic endothelial cell-printed (to represent soft tissue), human amniotic fluid-derived stem cell-printed (to represent hard tissue as they underwent osteogenic differentiation in vivo), and cell-free constructs (scaffold only). The constructs were subcutaneously implanted into athymic mice and regularly monitored using a magnetic resonance imaging (MRI) scanner. The three tissue construct types showed distinct image contrast characteristics due to the different tissue microstructures and biochemical compositions at various time points. In addition, changes in tissue microvasculature were examined with dynamic perfusion MRI. These results indicate that high resolution MRI is a promising method for noninvasive, long-term monitoring of the status of cell-printed construct growth, differentiation, and vascularization.
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April 2008
Research Papers
Characterization of Cell Constructs Generated With Inkjet Printing Technology Using In Vivo Magnetic Resonance Imaging
Tao Xu,
Tao Xu
Wake Forest Institute for Regenerative Medicine,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157
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John Olson,
John Olson
Center for Biomolecular Imaging,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157
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Weixin Zhao,
Weixin Zhao
Wake Forest Institute for Regenerative Medicine,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157
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Anthony Atala,
Anthony Atala
Wake Forest Institute for Regenerative Medicine,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157
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Jian-Ming Zhu,
Jian-Ming Zhu
Wake Forest Institute for Regenerative Medicine,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157; Department of Radiation Oncology, Wake Forest University Health Sciences
, Winston-Salem, NC 27157
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James J. Yoo
James J. Yoo
Wake Forest Institute for Regenerative Medicine,
e-mail: jyoo@wfubmc
Wake Forest University Health Sciences
, Winston-Salem, NC 27157
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Tao Xu
Wake Forest Institute for Regenerative Medicine,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157
John Olson
Center for Biomolecular Imaging,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157
Weixin Zhao
Wake Forest Institute for Regenerative Medicine,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157
Anthony Atala
Wake Forest Institute for Regenerative Medicine,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157
Jian-Ming Zhu
Wake Forest Institute for Regenerative Medicine,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157; Department of Radiation Oncology, Wake Forest University Health Sciences
, Winston-Salem, NC 27157
James J. Yoo
Wake Forest Institute for Regenerative Medicine,
Wake Forest University Health Sciences
, Winston-Salem, NC 27157e-mail: jyoo@wfubmc
J. Manuf. Sci. Eng. Apr 2008, 130(2): 021013 (7 pages)
Published Online: April 2, 2008
Article history
Received:
December 14, 2007
Revised:
January 11, 2008
Published:
April 2, 2008
Citation
Xu, T., Olson, J., Zhao, W., Atala, A., Zhu, J., and Yoo, J. J. (April 2, 2008). "Characterization of Cell Constructs Generated With Inkjet Printing Technology Using In Vivo Magnetic Resonance Imaging." ASME. J. Manuf. Sci. Eng. April 2008; 130(2): 021013. https://doi.org/10.1115/1.2902857
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