In order to explore the fluid flow and heat transfer features of supercritical fluids used in Brayton cycle for waste-heat utilization of marine gas turbines, the effects of ocean rolling motion on thermo-fluidic characteristics of supercritical carbon dioxide (SCO2) in a circular tube are computationally investigated based on a verified turbulence model. It can be found that at a given rolling period, compared to that under static condition, the time-averaged heat transfer capacity is improved by 7.9%, but the onset of the heat transfer recovery is delayed so that the range of the heat transfer deterioration becomes widened. Under the action of the inertial forces, the heat exchange between cooler/denser and warmer/lighter fluids is enhanced, a secondary circulation formed at t/tc = 0.325 and the maximum improvement of section-averaged heat transfer coefficient is 71% at this time. For various periods, the variation trend of time-averaged heat transfer coefficient for SCO2 shows a parabolic, which is distinguishing from conventional fluids. A polarization phenomenon for instantaneous thermal performance can be observed under severe rolling. With rise of the layout height, the time-average heat transfer performance of tube increases monotonously, and the maximum increment is 10.64% in study range.