While numerous applied studies have successfully demonstrated the feasibility of unsteady cooling solutions, a consensus has yet to be reached on the local instantaneous conditions that result in heat transfer enhancement. This work aims to experimentally validate a recent analytical solution (on a local time-dependent basis) for the common flow condition of a fully developed incompressible pulsating flow in a uniformly heated vessel. The experimental setup is found to approximate the ideal constant heat flux boundary condition well, especially for the decoupled unsteady scenario where the amplitude of the most significant secondary contributions (capacitance and lateral conduction) amounts to 1.2% and 0.2% of the generated heat flux, respectively. Overall, the experimental measurements for temperature and heat flux oscillations are found to coincide well with a recent analytical solution to the energy equation by the authors. Furthermore, local time-dependent heat flux enhancements and degradations are observed to be qualitatively similar to those of wall shear stress from a previous study, suggesting that the thermal performance is indeed influenced by hydrodynamic behavior.