Ultrafast cooling is the key to successful cell vitrification cryopreservation of lower concentration cryoprotective solution. This research develops a cell cryopreservation methodology which utilizes thin film evaporation and achieves vitrification of relatively low concentration cryoprotectant with an ultrafast cooling rate. Experimental results show that the average cooling rate of dimethylsulfoxide (DMSO) cryoprotective solution reaches 150,000 °C/min in a temperature range from 10 °C to −180 °C. The ultrafast cooling rate can remarkably improve the vitrification tendencies of the cryoprotective solution. This methodology opens the possibility for more successful cell vitrification cryopreservation.
An Ultrafast Vitrification Method for Cell Cryopreservation
Presented at the 2016 ASME 5th Micro/Nanoscale Heat & Mass Transfer International Conference. Paper No. MNHMT2016-6319.
Contributed by the Heat Transfer Division of ASME for publication in the JOURNAL OF HEAT TRANSFER. Manuscript received March 17, 2016; final manuscript received May 23, 2017; published online August 16, 2017. Assoc. Editor: Chun Yang.
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Su, F., Zhao, N., Deng, Y., and Ma, H. (August 16, 2017). "An Ultrafast Vitrification Method for Cell Cryopreservation." ASME. J. Heat Transfer. January 2018; 140(1): 012001. https://doi.org/10.1115/1.4037327
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