Of interest is the accurate measurement of the enhanced thermal conductivity of certain nanofluids free from the impact of natural convection. Owing to its simplicity, wide range of applicability and short response time, the transient hot-wire method (THWM) is frequently used to measure the thermal conductivity of fluids. In order to gain a sufficiently high accuracy, special care should be taken to assure that each measurement is not affected by initial heat supply delay, natural convection, and signal noise. In this study, it was found that there is a temperature limit when using THWM due to the incipience of natural convection. The results imply that the temperature-dependence of the thermal conductivity enhancement observed by other researchers might be misleading when ignoring the impact of natural convection; hence, it could not be used as supporting evidence of the effectiveness of micromixing due to Brownian motion. Thus, it is recommended that researchers report how they keep the impact of the natural convection negligible and check the integrity of their measurements in the future researches.

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