Chelating agent solutions have been proposed as effective fluids for enhancing oil production. Different recovery mechanisms are reported for increasing the oil recovery during chelating agent flooding. The aims of this work are to identify the possible recovery mechanisms during chelating agent flooding in carbonate reservoirs and to investigate the in situ CO 2 generation as a potential recovery mechanism during the injection of chelating agent solutions into carbonate reservoirs. The contribution of CO 2 on enhancing the oil recovery was determined using experimental measurements and analytical calculations. Several measurements were conducted to study the contribution of each mechanism on enhancing the oil recovery. Coreflooding tests, zeta potential measurements, CO 2 generation, and interfacial tension (IFT) experiments were carried out. Also, analytical models were utilized to determine the impact of the injected chemicals on reducing the capillary pressure and improving the flow conditions. In flooding tests, two chemicals (EDTA and GLDA) were injected in a sequential mode and the chemical concentration was increased gradually. In addition, a comparative study was performed to evaluate the effectiveness of EDTA and GLDA solutions to enhance oil recovery. Several parameters were investigated in this paper including incremental oil recovery, in situ CO 2 generation, hydrocarbon swelling, IFT, wettability alteration, permeability enhancement, productivity index, and chemical cost. The obtained results show that GLDA chelating agent has better performance than EDTA solutions for enhancing the oil recovery when the same concentrations are used. Also, the in situ generation of CO 2 shows a significant impact on improving the oil recovery from carbonate reservoirs during chelating agent flooding. In the literature, the reported recovery mechanisms of using chelating agents are the IFT reduction, wettability alteration, and rock dissolution. Based on this work, injecting chelating agent solutions at low pH can lead to involve additional recovery mechanisms due to the CO 2 generation, the additional mechanisms are hydrocarbon swelling, viscosity and density reduction, and oil vaporization.