The shortage of fossil fuels has been growing at a faster pace every year, which is the reason why it is necessary to switch to alternative fuels without making significant modifications to diesel engines. Because it satisfies the standards, biodiesel can serve as an efficient alternative to fuels derived from petroleum. Although biofuels may be produced from a wide variety of edible sources, the development of biofuels from non-edible sources has been more beneficial in terms of both the economical approach and the performance of the fuel. The addition of the non-carbonous source of nanoparticles is able to significantly increase the performance of the engine. The experimental investigation was carried out in a variety of chicken waste biodiesel blends that also contained titanium oxides at the rate of 50 ppm and 100 ppm. The used biodiesel blends were CWB10% (90% diesel + 10% chicken biodiesel), CWB20% (80% diesel + 20% chicken biodiesel), and nanoparticles were added at the rate of 5 ppm and 10 ppm. It was necessary to estimate the performance, emission, and combustion parameters of the utilized chicken waste biodiesel in order to arrive at an accurate assessment of its quality as a fuel. When comparing the outcomes of using biodiesel with those of using nanoparticles at two different ppm concentrations, the pure diesel results were utilized as a point of comparison. According to the findings, the application of biodiesel led to results that were just average. However, when titanium oxide was included in the mix, the outcomes of the experiment were much enhanced.