A simulated fuel storage tank was used to study biodeterioration of linear low-density polyethylene over 100 days. The system consisted of a water layer inoculated with microorganisms and a fuel layer of diesel/biodiesel. Biodeterioration was characterized measuring: biofilm growth, surface chemistry, crystallinity, and topography. Results showed greater accumulation of biofilm at higher biodiesel concentrations. Polyethylene biodegradation measured as consumption of oxidized species, increase in contact angle with water and reduction in electron donor groups was observed in all samples and was slightly higher in biodiesel-rich fuels. Topography changes and weight loss showed that microbial penetration in the polymer was superficial.

References

References
1.
Sørensen
,
G.
,
Pedersen
,
D. V.
,
Nørgaard
,
A. K.
,
Sørensen
,
K. B.
, and
Nygaard
,
S. D.
,
2011
, “
Microbial Growth Studies in Biodiesel Blends
,”
Bioresour. Technol.
,
102
(
8
), pp.
5259
5264
.10.1016/j.biortech.2011.02.017
2.
Qiu
,
Z.
,
Zhao
,
L.
, and
Weatherley
,
L.
,
2010
, “
Process Intensification Technologies in Continuous Biodiesel Production
,”
Chem. Eng. Process.: Process Intensif.
,
49
(
4
), pp.
323
330
.10.1016/j.cep.2010.03.005
3.
Hasheminejad
,
M.
,
Tabatabaei
,
M.
,
Mansourpanah
,
Y.
,
Khatami
,
M.
, and
Javani
,
A.
,
2011
, “
Upstream and Downstream Strategies to Economize Biodiesel Production
,”
Bioresour. Technol.
,
102
(
2
), pp.
461
468
.10.1016/j.biortech.2010.09.094
4.
Meher
,
L.
,
Vidyasagar
,
D.
, and
Naik
,
S.
,
2006
, “
Technical Aspects of Biodiesel Production by Transesterification—A Review
,”
Renewable Sustainable Energy. Rev.
,
10
(
3
), pp.
248
268
.10.1016/j.rser.2004.09.002
5.
Thompson
,
M. R.
,
Mu
,
B.
,
Ewaschuk
,
C. M.
,
Cai
,
Y.
,
Oxby
,
K. J.
, and
Vlachopoulos
,
J.
,
2013
, “
Long Term Storage of Biodiesel/Petrol Diesel Blends in Polyethylene Fuel Tanks
,”
Fuel
,
108
, pp.
771
779
.10.1016/j.fuel.2013.02.040
6.
Maru
,
M. M.
,
Lucchese
,
M. M.
,
Legnani
,
C.
,
Quirino
,
W. G.
,
Balbo
,
A.
,
Aranha
,
I. B.
,
Costa
,
L. T.
,
Vilani
,
C.
,
de Sena
,
L. Á.
,
Damasceno
,
J. C.
,
dos Santos Cruz
,
T.
,
Lidízio
,
L. R.
,
Ferreira e Silva
,
R.
,
Jorio
,
A.
, and
Achete
,
C. A.
,
2009
, “
Biodiesel Compatibility With Carbon Steel and HDPE Parts
,”
Fuel Process. Technol.
,
90
(
9
), pp.
1175
1182
.10.1016/j.fuproc.2009.05.014
7.
Richaud
,
E.
,
Flaconnèche
,
B.
, and
Verdu
,
J.
,
2012
, “
Biodiesel Permeability in Polyethylene
,”
Polym. Test.
,
31
(
8
), pp.
1070
1076
.10.1016/j.polymertesting.2012.08.003
8.
Klofutar
,
B.
, and
Golob
,
J.
,
2007
, “
Microorganisms in Diesel and in Biodiesel Fuels
,”
Acta Chim. Slov.
,
54
(
3
), pp.
744
748
.
9.
Gaylarde
,
C.
,
Bento
,
F.
, and
Kelley
,
J.
,
1999
, “
Microbial Contamination of Stored Hydrocarbon Fuels and Its Control
,”
Rev. Microbiol.
,
30
(1), pp.
1
10
.10.1590/S0001-37141999000100001
10.
Restrepo-Flórez
,
J.-M.
,
Bassi
,
A.
,
Rehmann
,
L.
, and
Thompson
,
M. R.
,
2013
, “
Effect of Biodiesel Addition on Microbial Community Structure in a Simulated Fuel Storage System
,”
Bioresour. Technol.
,
147
, pp.
456
463
.10.1016/j.biortech.2013.08.068
11.
Albertson
,
A. C.
,
Barensted
,
C.
,
Karlsson
,
S.
, and
Torbjorn
,
L.
,
1995
, “
Degradation Product Pattern and Morphology Changes as Means to Differentiate Abiotically and Biotically Aged Degradable Polyethylene
,”
Polymer
,
36
(
16
), pp.
3075
3083
.10.1016/0032-3861(95)97868-G
12.
Albersson
,
A.
, and
Karlsson
,
S.
,
1990
, “
The Influence of Biotic and Abiotic Environments on the Degradation of Polyethylene
,”
Prog. Polym. Sci.
,
15
(
2
), pp.
177
192
.10.1016/0079-6700(90)90027-X
13.
Santo
,
M.
,
Weitsman
,
R.
, and
Sivan
,
A.
,
2012
, “
The Role of the Copper-Binding Enzyme—Laccase—in the Biodegradation of Polyethylene by the Actinomycete Rhodococcus ruber
,”
Int. Biodeterior. Biodegrad.
,
84
, pp.
204
210
.10.1016/j.ibiod.2012.03.001
14.
Balasubramanian
,
V.
,
Natarajan
,
K.
,
Hemambika
,
B.
,
Ramesh
,
N.
,
Sumathi
,
C. S.
,
Kottaimuthu
,
R.
, and
Rajesh
,
V.
,
2010
, “
High-Density Polyethylene (HDPE)-Degrading Potential Bacteria From Marine Ecosystem of Gulf of Mannar, India
,”
Lett. Appl. Microbiol.
,
51
(
2
), pp.
205
211
.10.1111/j.1472-765X.2010.02883.x
15.
Hadad
,
D.
,
Geresh
,
S.
, and
Sivan
,
A.
,
2005
, “
Biodegradation of Polyethylene by the Thermophilic Bacterium Brevibacillus borstelensis
,”
J. Appl. Microbiol.
,
98
(
5
), pp.
1093
1100
.10.1111/j.1365-2672.2005.02553.x
16.
Sudhakar
,
M.
,
Doble
,
M.
,
Murthy
,
P. S.
, and
Venkatesan
,
R.
,
2008
, “
Marine Microbe-Mediated Biodegradation of Low- and High-Density Polyethylenes
,”
Int. Biodeterior. Biodegrad.
,
61
(
3
), pp.
203
213
.10.1016/j.ibiod.2007.07.011
17.
Chiellini
,
E.
,
Corti
,
A.
, and
Swift
,
G.
,
2003
, “
Biodegradation of Thermally-Oxidized, Fragmented Low-Density Polyethylenes
,”
Polym. Degrad. Stab.
,
81
(
2
), pp.
341
351
.10.1016/S0141-3910(03)00105-8
18.
Volke-Sepulveda
,
T.
,
Saucedo-Castañeda
,
G.
,
Gutierrez-Rojas
,
M.
,
Manzur
,
A.
, and
Favela-Torres
,
E.
,
2002
, “
Thermally Treated Low Density Polyethylene Biodegradation by Penicillium pinophilum and Aspergillus niger
,”
J. Appl. Polym. Sci.
,
83
(
2
), pp.
305
314
.10.1002/app.2245
19.
Artham
,
T.
,
Sudhakar
,
M.
,
Venkatesan
,
R.
,
Madhavan
,
C.
,
Murty
,
K. V. G. K.
, and
Doble
,
M.
,
2009
, “
Biofouling and Stability of Synthetic Polymers in Sea Water
,”
Int. Biodeterior. Biodegrad.
,
63
(
7
), pp.
884
890
.10.1016/j.ibiod.2009.03.003
20.
Gilan
,
I.
,
Hadar
,
Y.
, and
Sivan
,
A.
,
2004
, “
Colonization, Biofilm Formation and Biodegradation of Polyethylene by a Strain of Rhodococcus Ruber
,”
Appl. Microbiol. Biotechnol.
,
65
(
1
), pp.
97
104
.10.1007/s00253-004-1584-8
21.
Nowak
,
B.
,
Pająk
,
J.
,
Drozd-Bratkowicz
,
M.
, and
Rymarz
,
G.
,
2011
, “
Microorganisms Participating in the Biodegradation of Modified Polyethylene Films in Different Soils Under Laboratory Conditions
,”
Int. Biodeterior. Biodegrad.
,
65
(
6
), pp.
757
767
.10.1016/j.ibiod.2011.04.007
22.
Fontanella
,
S.
,
Bonhomme
,
S.
,
Koutny
,
M.
,
Husarova
,
L.
,
Brusson
,
J.-M.
,
Courdavault
,
J.-P.
,
Pitteri
,
S.
,
Samuel
,
G.
,
Pichon
,
G.
,
Lemaire
,
J.
, and
Delort
,
A.-M.
,
2010
, “
Comparison of the Biodegradability of Various Polyethylene Films Containing Pro-Oxidant Additives
,”
Polym. Degrad. Stab.
,
95
(
6
), pp.
1011
1021
.10.1016/j.polymdegradstab.2010.03.009
23.
Roy
,
P. K.
,
Titus
,
S.
,
Surekha
,
P.
,
Tulsi
,
E.
,
Deshmukh
,
C.
, and
Rajagopal
,
C.
,
2008
, “
Degradation of Abiotically Aged LDPE Films Containing Pro-Oxidant by Bacterial Consortium
,”
Polym. Degrad. Stab.
,
93
(
10
), pp.
1917
1922
.10.1016/j.polymdegradstab.2008.07.016
24.
Pramila
,
R.
, and
Ramesh
,
K.
,
2011
, “
Biodegradation of Low Density Polyethylene (LDPE) by Fungi Isolated From Marine Water—A SEM Analysis
,”
African J. Microbiol Res.
,
5
(
28
), pp.
5013
5018
.10.5897/AJMR11.670
25.
Sivan
,
A.
,
Szanto
,
M.
, and
Pavlov
,
V.
,
2006
, “
Biofilm Development of the Polyethylene-Degrading Bacterium Rhodococcus ruber
,”
Appl. Microbiol. Biotechnol.
,
72
(
2
), pp.
346
352
.10.1007/s00253-005-0259-4
26.
Koutny
,
M.
,
Sancelme
,
M.
,
Dabin
,
C.
,
Pichon
,
N.
,
Delort
,
A.-M.
, and
Lemaire
,
J.
,
2006
, “
Acquired Biodegradability of Polyethylenes Containing Pro-Oxidant Additives
,”
Polym. Degrad. Stab.
,
91
(
7
), pp.
1495
1503
.10.1016/j.polymdegradstab.2005.10.007
27.
Albertsson
,
A.-C.
,
Andersson
,
S. O.
, and
Karlsson
,
S.
,
1987
, “
The Mechanism of Biodegradation of Polyethylene
,”
Polym. Degrad. Stab.
,
18
(
1
), pp.
73
87
.10.1016/0141-3910(87)90084-X
28.
Albertson
,
A.
,
1980
, “
The Shape of the Biodegradation Curve for Low and High Density Polyethenes in Prolonged Series of Experiments
,”
Eur. Polym. J.
,
16
(
7
), pp.
623
630
.10.1016/0014-3057(80)90100-7
29.
Rojo
,
F.
,
2010
,
Handbook of Hydrocarbon and Lipid Microbiology
,
Springer
,
Berlin, Germany
.
30.
Karlsson
,
S.
,
Ljungquist
,
O.
, and
Albertsson
,
A.-C.
,
1988
, “
Biodegradation of Polyethylene and the Influence of Surfactants
,”
Polym. Degrad. Stab.
,
21
(
3
), pp.
237
250
.10.1016/0141-3910(88)90030-4
31.
Farahani
,
M.
,
Pagé
,
D. J. Y. S.
,
Turingia
,
M. P.
, and
Tucker
,
B. D.
,
2009
, “
Biodegradability and Degrading Microbes of Low-Density Polyethylene
,”
ASME J. Energy Resour. Technol.
,
131
(
4
), p.
041801
.10.1115/1.4000177
32.
Pegram
,
J. E.
, and
Andrady
,
A. L.
,
1989
, “
Outdoor Weathering of Selected Polymeric Materials Under Marine Exposure Conditions
,”
Polym. Degrad. Stab.
,
26
(
4
), pp.
333
345
.10.1016/0141-3910(89)90112-2
33.
Lobelle
,
D.
, and
Cunliffe
,
M.
,
2011
, “
Early Microbial Biofilm Formation on Marine Plastic Debris
,”
Mar. Pollut. Bull.
,
62
(
1
), pp.
197
200
.10.1016/j.marpolbul.2010.10.013
34.
Mumtaz
,
T.
,
Khan
,
M. R.
, and
Hassan
,
M. A.
,
2010
, “
Study of Environmental Biodegradation of LDPE Films in Soil Using Optical and Scanning Electron Microscopy
,”
Micron
,
41
(
5
), pp.
430
438
.10.1016/j.micron.2010.02.008
35.
Orhan
,
Y.
, and
Hanife
,
B.
,
2000
, “
Enhancement of Biodegradability of Disposable Polyethylene in Controlled Biological Soil
,”
Int. Biodeterior. Biodegrad.
,
45
(
1
), pp.
49
55
.10.1016/S0964-8305(00)00048-2
36.
Richard
,
J.
, and
Vogel
,
T.
,
1999
, “
Characterization of a Soil Bacterial Consortium Capable of Degrading Diesel Fuel
,”
Int. Biodeterior. Biodegrad.
,
44
(
2–3
), pp.
93
100
.10.1016/S0964-8305(99)00062-1
37.
Stepanovic
,
S.
,
Vukovic
,
D.
,
Dakic
,
I.
,
Savic
,
B.
, and
Svabic-Vlahovic
,
M.
,
2000
, “
A Modified Microtiter-Plate Test for Quantification of Staphylococcal Biofilm Formation
,”
J. Microbiol. Methods
,
40
(
2
), pp.
175
179
.10.1016/S0167-7012(00)00122-6
38.
Oss van
,
C. J.
,
2006
,
Interfacial Forces in Aqueous Media
,
CRC Press
,
Boca Raton
.
39.
Blaine
,
R. L.
,
2013
,
Determination of Polymer Crystallinity by DSC
,
TA Instruments
,
New Castle, DE
.
40.
Karcz
,
J.
,
Bernas
,
T.
,
Nowak
,
A.
,
Talik
,
E.
, and
Woznica
,
A.
,
2012
, “
Application of Lyophilization to Prepare the Nitrifying Bacterial Biofilm for Imaging With Scanning Electron Microscopy
,”
Scanning
,
34
(
1
), pp.
26
36
.10.1002/sca.20275
41.
Tribedi
,
P.
, and
Sil
,
A. K.
,
2013
, “
Low-Density Polyethylene Degradation by Pseudomonas SP. AKS2 Biofilm
,”
Environ. Sci. Pollut. Res. Int.
,
20
(
6
), pp.
4146
4153
.10.1007/s11356-012-1378-y
42.
Pompilio
,
A.
,
Piccolomini
,
R.
,
Picciani
,
C.
,
D'Antonio
,
D.
,
Savini
,
V.
, and
Di Bonaventura
,
G.
,
2008
, “
Factors Associated With Adherence to and Biofilm Formation on Polystyrene by Stenotrophomonas maltophilia: The Role of Cell Surface Hydrophobicity and Motility
,”
FEMS Microbiol. Lett.
,
287
(
1
), pp.
41
47
.10.1111/j.1574-6968.2008.01292.x
43.
Raghavan
,
D.
, and
Torma
,
A. E.
,
1992
, “
DSC and FTIR Characterization of Biodegradation of Polyethylene
,”
Polym. Eng. Sci.
,
32
(
6
), pp.
438
442
.10.1002/pen.760320609
44.
Manzur
,
A.
,
Limón-González
,
M.
, and
Favela-Torres
,
E.
,
2004
, “
Biodegradation of Physicochemically Treated LDPE by a Consortium of Filamentous Fungi
,”
J. Appl. Polym. Sci.
,
92
(
1
), pp.
265
271
.10.1002/app.13644
45.
Manzur
,
A.
,
Cuamatzi
,
F.
, and
Favela
,
E.
,
1997
, “
Effect of the Growth of Phanerochaete chrysosporium in a Blend of Low Density Polyethylene and Sugarcane Bagasse
,”
J. Appl. Polym. Sci.
,
66
(
1
), pp.
105
111
.10.1002/(SICI)1097-4628(19971003)66:1<105::AID-APP12>3.0.CO;2-0
46.
Muthukumar
,
T.
,
Aravinthan
,
A.
,
Lakshmi
,
K.
,
Venkatesan
,
R.
,
Vedaprakash
,
L.
, and
Doble
,
M.
,
2011
, “
Fouling and Stability of Polymers and Composites in Marine Environment
,”
Int. Biodeterior. Biodegrad.
,
65
(
2
), pp.
276
284
.10.1016/j.ibiod.2010.11.012
47.
Watanabe
,
T.
,
Ohtake
,
Y.
,
Asabe
,
H.
,
Murakami
,
N.
, and
Furukawa
,
M.
,
2009
, “
Biodegradability and Degrading Microbes of Low-Density Polyethylene
,”
J. Appl. Polym. Sci.
,
111
(
1
), pp.
551
559
.10.1002/app.29102
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