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BioNano Monographs

GFP Whole Cell Microbial Biosensors: Scale-up and Scale-down Effects on Biopharmaceutical Processes

Editor
F. Delvigne
F. Delvigne
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A. Brognaux
A. Brognaux
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S. Han
S. Han
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S. J. Sørensen
S. J. Sørensen
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P. Thonart
P. Thonart
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ISBN:
9780791860090
No. of Pages:
60
Publisher:
ASME Press
Publication date:
2012

A series of scale-down experiments have been carried out by our team. During these experiments, different GFP transcriptional reporters have been used in order to characterize the response of microbial cells in front of the environmental fluctuations perceived during the cultures. These transcriptional reporters have been constructed form different natural promoters involving either the general stress response, the response to nutrient limitation or a dependency to the growth rate of the strain. The experimental results are reported in the following section, but it is first useful to specify the cultivation conditions used to carry out these experiments.

3.1 General stress response in scale-down bioreactors: rpoS::GFP
3.2 Use of destabilized GFP variants
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