International Conference on Computer Technology and Development, 3rd (ICCTD 2011)
135 The Complementary DNA Segment Cloning and Bioinformatics Analysis of INTS2 Gene in Goose
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To clone the INTS2 (Integrator complex subunit 2) cDNA segment in goose, we isolated total RNA from goose liver. The 488bp cDNA segment was amplified by the reverse transcription polymerase chain reaction (RT-PCR). The obtained segment had 3 open reading frames (ORF) and two of them are separately 100% and 99% relative to the sequences of chicken. One of the segment contains 113 amino acids, 63.7% of them form helix and 36.3% loop. And the other 45AA segment contains 22.2% helix, 17.8% strand and 60% loop. Comparison analysis indicated that the nucleotide sequence and amino acid sequence of INTS2 gene segment were highly homologous to some species reported, such as Gallus gallus, Taeniopygia guttata, Ornithorhynchus anatinus ect.. Homology between Sichuan White Goose and Gallus gallus was 94%, and Taeniopygia guttata 88%. We confirmed the sequence to be segment of INTS2 gene.