Bacteriophage T4 Tail Fibers as a Basis for Structured Assemblies
As shown in the results presented here, some of our hypotheses concerning the ability to modify particle segments of the P37 segment of the tail fiber were correct. We found or constructed tail fibers of altered length and inserted several peptide segments that added novel functionality. Clearly particular functional domains of gp 37 can be modified without affecting neighboring domains. Work by other researchers in the Goldberg lab demonstrated other binding element insertions. However, moving beyond simple attachment sites has proven more problematic. Functional attachment of nanoparticles, as described above, proved more difficult. In work not shown here, we did attempt to create hybrid and chimeric hybrid assembly proteins as described in Section 2.2. While we had some limited results on hybrid construction, no evidence for chimeric hybrid formation was obtained.