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BioNano Monographs

Bacteriophage T4 Tail Fibers as a Basis for Structured Assemblies

Editor
Paul Hyman
Paul Hyman
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Timothy Harrah
Timothy Harrah
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ISBN:
9780791860373
No. of Pages:
100
Publisher:
ASME Press
Publication date:
2014

While much of our work was focused on altering tail fiber proteins to enhance existing properties or add novel ones, we also addressed the issue of large scale production of assembled tail fibers. Several methods for the purification of the long tail fiber and its component half fibers in phage infected lysates have been reported previously (Cerritelli et al., 1996; Earnshaw et al., 1979; Imada and Tsugita, 1970; Imada and Tsugita, 1972; Selivanov et al., 1987; Ward and Wood, 1968; Ward et al., 1970). However, each of these methods for LTF purification relied on serial column based chromatographic steps that can be complex, expensive and relatively inefficient. As our area of interest revolved around the manipulation of LTF, and since production of large quantities of LTF was essential to the further study of LTF structure and its use as a nanomaterial, we developed a non-chromatographic purification scheme that can be used for the rapid, efficient and economical purification of natively folded LTFs.

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