Skip to Main Content
ASME Press Select Proceedings

International Conference on Computer Research and Development, 5th (ICCRD 2013)

Editor
Fan Yama
Fan Yama
Search for other works by this author on:
ISBN:
9780791860182
No. of Pages:
278
Publisher:
ASME Press
Publication date:
2013

The objectives of this research are to determine the phylogenetic relationships among populations of Macaca fascicularis in Malaysia. This research involved 14 individuals of Macaca with locus Cytochrome C Oxidase subunit I (COI). DNA was extracted from blood, tissue and liver. Nine individuals which are species M. fascicularis, M. nemestrina, Trachypi thecus cristatus and Trachypithecus obscurus were used to generate phylogenetic relationships among populations of M. fascicularis. COI region sequences of mtDNA have been obtained in size of 700 bp from PCR process. Then, by using the genetic softwares which are Bioedit, MEGA, ClustalW, PAUP and MrBayes, the samples were aligned and analyzed. The phylogeny tree topology was constructed using the Neighbor-Joining, and Maximum Parsimony methods. The phylogeny tree topology shows that M. fascicularis do not segregates according to the theoretical phyleogeography in Malaysia. This is because the sample from Negeri Sembilan has merged together into the samples from Perak and Penang in one clade. However, M. fascicularis has successfully separated from the other species and genus thus form monophyletic group.

This content is only available via PDF.
Close Modal
This Feature Is Available To Subscribers Only

Sign In or Create an Account

Close Modal
Close Modal