47 Bioinformatics Analysis of the Small Subunit of Ribonucleotide Reductase Encoded by UL40 Gene from Duck Plague Virus CHv Strain

Duck plague virus (DPV) is an alphaherpesvirus, which causes an acute, contagious, and fatal disease. In the present article, the small subunit of ribonucleotide reductase (R2), a 355 amino acid polypeptide, which was encoded by DPV-CHv UL40 gene (GeneBank Accession No. EU071039), was analysed through bioinformatics methods. The results revealing that there is a transmembrane region, and its molecular mass is 40.6812 Kda, isoelectric point (IP) is 4.65. The phylogenetic tree shows that DPV-CHv and AnHV-1 are a separate branch, that is to say, DPV may be an osculant example among the alphaherpesvirus genera. In addition, the analysis of the physico-chemical properties demonstrates that R2 has 1 potential N-glycosylation site, 10 main antigenic determinants, and 19 phosphorylation sites. And the subcellular localization analysis indicates that R2 is mainly located in cytoplasmic, nuclear, and mitochondrial. In conclusion, all those consequences are valuable for further resarches on more accurate molecular characteristic of DPV-CHv R2 and the complete DPV genome.