Molecularly imprinted polymer on chip was prepared for the detection of chloramphenicol by photopolymerization method using diethylaminoethyl methacrylate as the functional monomer. This method was developed and characterized for the first time in this study. The imprinting effect of the MIPs was assessed by the chromatographic method, which demonstrated that the MIPs had better chromatographic behavior and selectivity than those of the corresponding NIPs. A molecularly imprinted polymer on microchip LCMS/MS on chip method is reported for determination of CAP residue in milk and honey samples. It relies on dispersive CAP-MIP solid-phase micro extraction for clean-up of the samples, and HPLC on a CAP-MIP monolith on chip with ESI detection for analysis. Linear calibration curve was obtained over the concentration range of 7.75110^1’4–6.20110^1’31…1mole with the correlation coefficient of 0.9900. The detection limit and the quantification limit were found to be 2.10110^1’41…1mole and 6.80110^1’41…1mole⁠, respectively. The precisions of the proposed method were found to be 0.21, 2.71 and 1.34%, respectively. The mean percentage recoveries of CAP were found to be in the ranges of 95.18–96.85, indicating that the proposed method could provide acceptable extraction efficiency and recovery of this method was good.