Copper anodic slime which is an insoluble residue of copper electro-refining process contains valuable elements such as gold, silver, selenium and tellurium and is a byproduct of copper ingot production. Copper anodic slime contains 0.12% gold approximately. The common methods to extract gold from copper anodic slime are hydrometallurgical processes. The aim of this research is extraction and recovery of gold from copper anodic slimes by means of magnetite nanoparticles. For this, copper anodic slime was leached with nitric acid by which elements like silver, copper and iron dissolved while gold remained in the solid phase. The remained solid phase were leached by Thiourea solution and as a result gold were transferred to the solution phase as Au(CS(NH₂))⁺² positive complex. Magnetite nanoparticles were synthesized by coprecipitation method with iron(II) and iron(III) ions in the presence of ammonia and were silica-coated by the famous Stober method. In higher pHs than pH(pzc), the nanoparticles were added to the gold solution and the gold complex was absorbed on the nanoparticles due to their negative surface charge. Finally by adding various amounts of HCl, the gold complexes were desorbed and gold entered the solution as Au⁺. According to the results, up to 70% of gold was desorbed and entered the solution.