Postmeniscectomy osteoarthritis (OA) is hypothesized to be the consequence of abnormal mechanical conditions, but the relationship between postsurgical alterations in articular cartilage strain and in vivo biomechanical/biochemical changes in articular cartilage is unclear. We hypothesized that spatial variations in cartilage nominal strain (percentile thickness change) would correlate with previously reported in vivo articular cartilage property changes following meniscectomy. Cadevaric sheep knees were loaded in cyclic compression which was previously developed to mimic normal sheep gait, while a 4.7 T magnetic resonance imaging (MRI) imaged the whole joint. 3D cartilage strain maps were compared with in vivo sheep studies that described postmeniscectomy changes in shear modulus, phase lag, proteoglycan content and collagen organization/content in the articular cartilage. The area of articular cartilage experiencing high (overloaded) and low (underloaded) strain was significantly increased in the meniscectomized tibial compartment by 10% and 25%, respectively, while no significant changes were found in the nonmeniscectomized compartment. The overloaded and underloaded regions of articular cartilage in our in vitro specimens correlated with regions of in vivo shear modulus reduction. Glycosaminoglycans (GAG) content only increased at the underloaded articular cartilage but decreased at the overloaded articular cartilage. No significant correlation was found in phase lag and collagen organization/content changes with the strain variation. Comparisons between postsurgical nominal strain and in vivo cartilage property changes suggest that both overloading and underloading after meniscectomy may directly damage the cartilage matrix stiffness (shear modulus). Disruption of superficial cartilage by overloading might be responsible for the proteoglycan (GAG) loss in the early stage of postmeniscectomy OA.

References

References
1.
Roos
,
H.
,
Lauren
,
M.
,
Adalberth
,
T.
,
Roos
,
E. M.
,
Jonsson
,
K.
, and
Lohmander
,
L. S.
,
1998
, “
Knee Osteoarthritis After Meniscectomy: Prevalence of Radiographic Changes After Twenty-One Years, Compared With Matched Controls
,”
Arthritis Rheum.
,
41
(
4
), pp.
687
693
.10.1002/1529-0131(199804)41:4<687::AID-ART16>3.0.CO;2-2
2.
Jorgensen
,
U.
,
Sonne-Holm
,
S.
,
Lauridsen
,
F.
, and
Rosenklint
,
A.
,
1987
, “
Long-Term Follow-Up of Meniscectomy in Athletes. A Prospective Longitudinal Study
,”
J. Bone Jt. Surg. Br.
,
69
(
1
), pp.
80
83
.
3.
Appleyard
,
R. C.
,
Burkhardt
,
D.
,
Ghosh
,
P.
,
Read
,
R.
,
Cake
,
M.
,
Swain
,
M. V.
, and
Murrell
,
G. A.
,
2003
, “
Topographical Analysis of the Structural, Biochemical and Dynamic Biomechanical Properties of Cartilage in an Ovine Model of Osteoarthritis
,”
Osteoarthritis Cartilage
,
11
(
1
), pp.
65
77
.10.1053/joca.2002.0867
4.
Oakley
,
S. P.
,
Lassere
,
M. N.
,
Portek
, I
.
,
Szomor
,
Z.
,
Ghosh
,
P.
,
Kirkham
,
B. W.
,
Murrell
,
G. A.
,
Wulf
,
S.
, and
Appleyard
,
R. C.
,
2004
, “
Biomechanical, Histologic and Macroscopic Assessment of Articular Cartilage in a Sheep Model of Osteoarthritis
,”
Osteoarthritis Cartilage
,
12
(
8
), pp.
667
679
.10.1016/j.joca.2004.05.006
5.
Chatain
,
F.
,
Adeleine
,
P.
,
Chambat
,
P.
,
Neyret
,
P.
, and
Societe Francaise
,
D. A.
,
2003
, “
A Comparative Study of Medial Versus Lateral Arthroscopic Partial Meniscectomy on Stable Knees: 10-Year Minimum Follow-Up
,”
Arthroscopy
,
19
(
8
), pp.
842
849
.10.1016/S0749-8063(03)00735-7
6.
Leroux
,
M. A.
,
Arokoski
,
J.
,
Vail
,
T. P.
,
Guilak
,
F.
,
Hyttinen
,
M. M.
,
Kiviranta
, I
.
, and
Setton
,
L. A.
,
2000
, “
Simultaneous Changes in the Mechanical Properties, Quantitative Collagen Organization, and Proteoglycan Concentration of Articular Cartilage Following Canine Meniscectomy
,”
J. Orthop. Res.
,
18
(
3
), pp.
383
92
.10.1002/jor.1100180309
7.
Borel
,
M.
,
Pastoureau
,
P.
,
Papon
,
J.
,
Madelmont
,
J. C.
,
Moins
,
N.
,
Maublant
,
J.
, and
Miot-Noirault
,
E.
,
2009
, “
Longitudinal Profiling of Articular Cartilage Degradation in Osteoarthritis by High-Resolution Magic Angle Spinning 1h Nmr Spectroscopy: Experimental Study in the Meniscectomized Guinea Pig Model
,”
J. Proteome Res.
,
8
(
5
), pp.
2594
2600
.10.1021/pr8009963
8.
Mckinley
,
T. O.
,
English
,
D. K.
, and
Bay
,
B. K.
,
2003
, “
Trabecular Bone Strain Changes Resulting from Partial and Complete Meniscectomy
,”
Clin. Orthop. Relat. Res.
,
407
, pp.
259
267
.10.1097/00003086-200302000-00035
9.
Wilson
,
W.
,
Van Rietbergen
,
B.
,
Van Donkelaar
,
C. C.
, and
Huiskes
,
R.
,
2003
, “
Pathways of Load-Induced Cartilage Damage Causing Cartilage Degeneration in the Knee After Meniscectomy
,”
J. Biomech.
,
36
(
6
), pp.
845
851
.10.1016/S0021-9290(03)00004-6
10.
Song
,
Y.
,
Greve
,
J. M.
,
Carter
,
D. R.
,
Koo
,
S.
, and
Giori
,
N. J.
,
2006
, “
Articular Cartilage Mr Imaging and Thickness Mapping of a Loaded Knee Joint Before and After Meniscectomy
,”
Osteoarthritis Cartilage
,
14
(
8
), pp.
728
737
.10.1016/j.joca.2006.01.011
11.
Song
,
Y.
,
Greve
,
J. M.
,
Carter
,
D. R.
, and
Giori
,
N. J.
,
2008
, “
Meniscectomy Alters the Dynamic Deformational Behavior and Cumulative Strain of Tibial Articular Cartilage in Knee Joints Subjected to Cyclic Loads
,”
Osteoarthritis Cartilage
,
16
(
12
), pp.
1545
1554
.10.1016/j.joca.2008.04.011
12.
Burkhardt
,
D.
,
Hwa
,
S. Y.
, and
Ghosh
,
P.
,
2001
, “
A Novel Microassay for the Quantitation of the Sulfated Glycosaminoglycan Content of Histological Sections: Its Application to Determine the Effects of Diacerhein on Cartilage in an Ovine Model of Osteoarthritis
,”
Osteoarthritis Cartilage
,
9
(
3
), pp.
238
247
.10.1053/joca.2000.0381
13.
Appleyard
,
R. C.
,
Ghosh
,
P.
, and
Swain
,
M. V.
,
1999
, “
Biomechanical, Histological and Immunohistological Studies of Patellar Cartilage in an Ovine Model of Osteoarthritis Induced by Lateral Meniscectomy
,”
Osteoarthritis Cartilage
,
7
(
3
), pp.
281
294
.10.1053/joca.1998.0202
14.
Hoch
,
D. H.
,
Grodzinsky
,
A. J.
,
Koob
,
T. J.
,
Albert
,
M. L.
, and
Eyre
,
D. R.
,
1983
, “
Early Changes in Material Properties of Rabbit Articular Cartilage After Meniscectomy
,”
J. Orthop. Res.
,
1
(
1
), pp.
4
12
.10.1002/jor.1100010102
15.
Chen
,
A. C.
,
Klisch
,
S. M.
,
Bae
,
W. C.
,
Temple
,
M. M.
,
Mcgowan
,
K. B.
,
Gratz
,
K. R.
,
Schumacher
,
B. L.
, and
Sah
,
R. L.
,
2004
, “
Mechanical Characterization of Native and Tissue-Engineered Cartilage
,”
Methods Mol. Med.
,
101
, pp.
157
190
.
16.
Appleyard
,
R.
,
2005
, private communication.
17.
Taylor
,
W. R.
,
Ehrig
,
R. M.
,
Heller
,
M. O.
,
Schell
,
H.
,
Seebeck
,
P.
, and
Duda
,
G. N.
,
2006
, “
Tibio-Femoral Joint Contact Forces in Sheep
,”
J. Biomech.
,
39
(
5
), pp.
791
798
.10.1016/j.jbiomech.2005.02.006
18.
Eckstein
,
F.
,
Lemberger
,
B.
,
Stammberger
,
T.
,
Englmeier
,
K. H.
, and
Reiser
,
M.
,
2000
, “
Patellar Cartilage Deformation In Vivo After Static Versus Dynamic Loading
,”
J. Biomech.
,
33
(
7
), pp.
819
825
.10.1016/S0021-9290(00)00034-8
19.
Herberhold
,
C.
,
Faber
,
S.
,
Stammberger
,
T.
,
Steinlechner
,
M.
,
Putz
,
R.
,
Englmeier
,
K. H.
,
Reiser
,
M.
, and
Eckstein
,
F.
,
1999
, “
In Situ Measurement of Articular Cartilage Deformation in Intact Femoropatellar Joints Under Static Loading
,”
J. Biomech.
,
32
(
12
), pp.
1287
1295
.10.1016/S0021-9290(99)00130-X
20.
Mosher
,
T. J.
,
Smith
,
H. E.
,
Collins
,
C.
,
Liu
,
Y.
,
Hancy
,
J.
,
Dardzinski
,
B. J.
, and
Smith
,
M. B.
,
2005
, “
Change in Knee Cartilage T2 at Mr Imaging After Running: A Feasibility Study
,”
Radiology
,
234
(
1
), pp.
245
249
.10.1148/radiol.2341040041
21.
Andriacchi
,
T. P.
,
1994
, “
Dynamics of Knee Malalignment
,”
Orthop. Clin. North Am.
,
25
(
3
), pp.
395
403
.
22.
Mundermann
,
A.
,
Dyrby
,
C. O.
,
Hurwitz
,
D. E.
,
Sharma
,
L.
, and
Andriacchi
,
T. P.
,
2004
, “
Potential Strategies to Reduce Medial Compartment Loading in Patients With Knee Osteoarthritis of Varying Severity: Reduced Walking Speed
,”
Arthritis Rheum.
,
50
(
4
), pp.
1172
1178
.10.1002/art.20132
23.
Hurwitz
,
D. E.
,
Ryals
,
A. B.
,
Case
,
J. P.
,
Block
,
J. A.
, and
Andriacchi
,
T. P.
,
2002
, “
The Knee Adduction Moment During Gait in Subjects With Knee Osteoarthritis is More Closely Correlated With Static Alignment Than Radiographic Disease Severity, Toe Out Angle and Pain
,”
J. Orthop. Res.
,
20
(
1
), pp.
101
107
.10.1016/S0736-0266(01)00081-X
24.
Hurwitz
,
D. E.
,
Ryals
,
A. R.
,
Block
,
J. A.
,
Sharma
,
L.
,
Schnitzer
,
T. J.
, and
Andriacchi
,
T. P.
,
2000
, “
Knee Pain and Joint Loading in Subjects With Osteoarthritis of the Knee
,”
J. Orthop. Res.
,
18
(
4
), pp.
572
579
.10.1002/jor.1100180409
25.
Hurwitz
,
D. E.
,
Sumner
,
D. R.
,
Andriacchi
,
T. P.
, and
Sugar
,
D. A.
,
1998
, “
Dynamic Knee Loads During Gait Predict Proximal Tibial Bone Distribution
,”
J. Biomech.
,
31
(
5
), pp.
423
430
.10.1016/S0021-9290(98)00028-1
26.
Caligaris
,
M.
,
Canal
,
C. E.
,
Ahmad
,
C. S.
,
Gardner
,
T. R.
, and
Ateshian
,
G. A.
,
2009
, “
Investigation of the Frictional Response of Osteoarthritic Human Tibiofemoral Joints and the Potential Beneficial Tribological Effect of Healthy Synovial Fluid
,”
Osteoarthritis Cartilage
,
17
(
10
), pp.
1327
1332
.10.1016/j.joca.2009.03.020
27.
Gleghorn
,
J. P.
,
Jones
,
A. R.
,
Flannery
,
C. R.
, and
Bonassar
,
L. J.
,
2009
, “
Boundary Mode Lubrication of Articular Cartilage by Recombinant Human Lubricin
,”
J. Orthop. Res.
,
27
(
6
), pp.
771
777
.10.1002/jor.20798
28.
Schmidt
,
T. A.
, and
Sah
,
R. L.
,
2007
, “
Effect of Synovial Fluid on Boundary Lubrication of Articular Cartilage
,”
Osteoarthritis Cartilage
,
15
(
1
), pp.
35
47
.10.1016/j.joca.2006.06.005
29.
Wong
,
B. L.
,
Bae
,
W. C.
,
Gratz
,
K. R.
, and
Sah
,
R. L.
,
2008
, “
Shear Deformation Kinematics During Cartilage Articulation: Effect of Lubrication, Degeneration, and Stress Relaxation
,”
Mol. Cell Biomech.
,
5
(
3
), pp.
197
206
.
You do not currently have access to this content.