Engineered tissues are commonly stretched or compressed (i.e., conditioned) during culture to stimulate extracellular matrix (ECM) production and to improve the mechanical properties of the growing construct. The relationships between mechanical stimulation and ECM remodeling, however, are complex, interdependent, and dynamic. Thus, theoretical models are required for understanding the underlying phenomena so that the conditioning process can be optimized to produce functional engineered tissues. Here, we continue our development of multiscale mechanical models by simulating the effect of cell tractions on developing isometric tension and redistributing forces in the surrounding fibers of a collagen gel embedded with explants. The model predicted patterns of fiber reorganization that were similar to those observed experimentally. Furthermore, the inclusion of cell compaction also changed the distribution of fiber strains in the gel compared to the acellular case, particularly in the regions around the cells where the highest strains were found.
Multiscale Mechanical Simulations of Cell Compacted Collagen Gels
Contributed by the Bioengineering Division of ASME for publication in the JOURNAL OF BIOMECHANICAL ENGINEERING. Manuscript received January 8, 2013; final manuscript received May 1, 2013; accepted manuscript posted May 8, 2013; published online June 11, 2013. Assoc. Editor: Keith Gooch.
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Aghvami, M., Barocas, V. H., and Sander, E. A. (June 11, 2013). "Multiscale Mechanical Simulations of Cell Compacted Collagen Gels." ASME. J Biomech Eng. July 2013; 135(7): 071004. https://doi.org/10.1115/1.4024460
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