Applying tissue-engineered cartilage in a clinical setting requires noninvasive evaluation to detect the maturity of the cartilage. Magnetic resonance imaging (MRI) of articular cartilage has been widely accepted and applied clinically in recent years. In this study, we evaluated the negative fixed-charge density (nFCD) of tissue-engineered cartilage using gadolinium-enhanced MRI and determined the relationship between nFCD and biomechanical properties. To reconstruct cartilage tissue, articular chondrocytes from bovine humeral heads were embedded in agarose gel and cultured in vitro for up to 4 weeks. The nFCD of the cartilage was determined using the MRI gadolinium exclusion method. The equilibrium modulus was determined using a compressive stress relaxation test, and the dynamic modulus was determined by a dynamic compression test. The equilibrium compressive modulus and dynamic modulus of the tissue-engineered cartilage increased with an increase in culture time. The nFCD value—as determined with the measurement using the MRI technique—increased with culture time. In the regression analysis, nFCD showed significant correlations with equilibrium compressive modulus and dynamic modulus. From these results, gadolinium-enhanced MRI measurements can serve as a useful predictor of the biomechanical properties of tissue-engineered cartilage.
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July 2010
Research Papers
Evaluation of Negative Fixed-Charge Density in Tissue-Engineered Cartilage by Quantitative MRI and Relationship With Biomechanical Properties
Shogo Miyata,
Shogo Miyata
Faculty of Science and Technology,
e-mail: miyata@mech.keio.ac.jp
Keio University
, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan
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Kazuhiro Homma,
Kazuhiro Homma
National Institute of Advanced Industrial Science and Technology (AIST)
, Ibaraki 305-8564, Japan
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Tomokazu Numano,
Tomokazu Numano
Tokyo Metropolitan University
, Tokyo 116-8551, Japan
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Tetsuya Tateishi,
Tetsuya Tateishi
National Institute for Material Science
, Ibaraki 305-0044, Japan
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Takashi Ushida
Takashi Ushida
Graduate School of Medicine,
University of Tokyo
, Tokyo 113-0033, Japan
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Shogo Miyata
Faculty of Science and Technology,
Keio University
, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japane-mail: miyata@mech.keio.ac.jp
Kazuhiro Homma
National Institute of Advanced Industrial Science and Technology (AIST)
, Ibaraki 305-8564, Japan
Tomokazu Numano
Tokyo Metropolitan University
, Tokyo 116-8551, Japan
Tetsuya Tateishi
National Institute for Material Science
, Ibaraki 305-0044, Japan
Takashi Ushida
Graduate School of Medicine,
University of Tokyo
, Tokyo 113-0033, JapanJ Biomech Eng. Jul 2010, 132(7): 071014 (6 pages)
Published Online: June 2, 2010
Article history
Received:
March 31, 2009
Revised:
March 2, 2010
Posted:
March 3, 2010
Published:
June 2, 2010
Online:
June 2, 2010
Citation
Miyata, S., Homma, K., Numano, T., Tateishi, T., and Ushida, T. (June 2, 2010). "Evaluation of Negative Fixed-Charge Density in Tissue-Engineered Cartilage by Quantitative MRI and Relationship With Biomechanical Properties." ASME. J Biomech Eng. July 2010; 132(7): 071014. https://doi.org/10.1115/1.4001369
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