The purpose of this study was to determine the kinetics of HSP70 expression in response to mild thermal stress. The rationale is to produce a basis for design of optimal heating methods to induce HSP70 expression for preconditioning in cardiac surgery. Bovine aortic endothelial cells were heated at for 0.5 to 5 hours followed by recovery for 1 to 48 hours. Quantitative analysis of western blot results showed HSP70 expression kinetics is a coupled function of heating temperature and time and of post-heating duration. Bimodal HSP70 expression kinetics were identified which may be an important cause of the “second window of protection” observed by other researchers.
Kinetics Study of Endogenous Heat Shock Protein 70 Expression
Contributed by the Bioengineering Division for publication in the JOURNAL OF BIOMECHANICAL ENGINEERING. Manuscript received by the Bioengineering Division, July 30, 2003; revision received August 11, 2003. Associate Editor: C. Dong.
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Wang , S., Diller, K. R., and Aggarwal, S. J. (January 9, 2004). "Kinetics Study of Endogenous Heat Shock Protein 70 Expression ." ASME. J Biomech Eng. December 2003; 125(6): 794–797. https://doi.org/10.1115/1.1632522
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