The present in-vitro study used bone marrow cell cultures and investigated the effects of cyclic pressure on osteoclastic bone resorption. Compared to control (cells maintained under static conditions), the number of tartrate resistant acid phosphatase (TRAP)-positive, osteoclastic cells was significantly lower when, immediately upon harvesting, bone marrow cells were exposed to cyclic pressure (10–40 kPa at 1.0 Hz). In contrast, once precursors in bone marrow cells differentiated into osteoclastic cells under static culture conditions for 7 days, subsequent exposure to the cyclic pressure of interest to the present study did not affect the number of osteoclastic cells. Most important, exposure of bone marrow cells to cyclic pressure for 1 h daily for 7 consecutive days resulted in significantly lower osteoclastic bone resorption and in lowered mRNA expression for interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α), cytokines that are known activators of osteoclast function. In addition to unique contributions to osteoclast physiology, the present study provided new evidence of a correlation between mechanical loading and bone homeostasis as well as insight into the molecular mechanisms of bone adaptation to mechanical loading, namely cytokine-mediated control of osteoclast functions.
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June 2002
Technical Papers
Effects of Cyclic Pressure on Bone Marrow Cell Cultures
Jiro Nagatomi,
Jiro Nagatomi
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590
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Bernard P. Arulanandam,
Bernard P. Arulanandam
Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY
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Dennis W. Metzger,
Dennis W. Metzger
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590
Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY
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Alain Meunier,
Alain Meunier
Faculte´ de Me´dicine Lariboisiere St-Louis, Universite D.Diderot, Paris, France
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Rena Bizios
Rena Bizios
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590
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Jiro Nagatomi
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590
Bernard P. Arulanandam
Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY
Dennis W. Metzger
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590
Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY
Alain Meunier
Faculte´ de Me´dicine Lariboisiere St-Louis, Universite D.Diderot, Paris, France
Rena Bizios
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590
Contributed by the Bioengineering Division for publication in the JOURNAL OF BIOMECHANICAL ENGINEERING. Manuscript received by the Bioengineering Division October 2, 2001; revised manuscript received February 5, 2002. Associate Editor: R. Vanderby, Jr.
J Biomech Eng. Jun 2002, 124(3): 308-314 (7 pages)
Published Online: May 21, 2002
Article history
Received:
October 2, 2001
Revised:
February 5, 2002
Online:
May 21, 2002
Citation
Nagatomi, J., Arulanandam, B. P., Metzger, D. W., Meunier, A., and Bizios, R. (May 21, 2002). "Effects of Cyclic Pressure on Bone Marrow Cell Cultures ." ASME. J Biomech Eng. June 2002; 124(3): 308–314. https://doi.org/10.1115/1.1468867
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