The study objectives were to quantify the time- and magnitude-dependence of flow-induced alignment in vascular smooth muscle cells (SMC) and to identify pathways related to the orientation process. Using an intensity gradient method, we demonstrated that SMC aligned in the direction perpendicular to applied shear stress, which contrasts with parallel alignment of endothelial cells under flow. SMC alignment varied with the magnitude of and exposure time to shear stress and is a continuous process that is dependent on calcium and cycloskeleton based mechanisms. A clear understanding and control of flow-induced SMC alignment will have implications for vascular tissue engineering.
Fluid Shear Stress-Induced Alignment of Cultured Vascular Smooth Muscle Cells
Contibuted by the Bioengineering Division for publication in the JOURNAL OF BIOMECHANICAL ENGINEERING. Manuscript received by the Bioengineering Division April 9, 2000; revised manuscript received October 2, 2001. Associate Editor: V. T. Turitto.
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Lee , A. A., Graham , D. A., Dela Cruz , S., Ratcliffe, A., and Karlon, W. J. (October 2, 2001). "Fluid Shear Stress-Induced Alignment of Cultured Vascular Smooth Muscle Cells ." ASME. J Biomech Eng. February 2002; 124(1): 37–43. https://doi.org/10.1115/1.1427697
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