An in vitro model for neural trauma was characterized and validated. The model is based on a novel device that is capable of applying high strain rate, homogeneous, and equibiaxial deformation to neural cells in culture. The deformation waveform is fully arbitrary and controlled via closed-loop feedback. Intracellular calcium alterations were recorded in real time throughout the imposed strain with an epifluorescent microscopy system. Peak change in recovery of and percent responding NG108-15 cells were shown to be dependent on strain rate to and magnitude (0.1 to 0.3 Green’s Strain). These measures were also shown to depend significantly on the interaction between strain rate and magnitude. This model for neural trauma is a robust system that can be used to investigate the cellular tolerance and response to traumatic brain injury.
An in Vitro Model of Neural Trauma: Device Characterization and Calcium Response to Mechanical Stretch
Contributed by the Bioengineering Division for publication in the JOURNAL OF BIOMECHANICAL ENGINEERING. Manuscript received by the Bioengineering Division December 27, 1999; revised manuscript received January 11, 2001. Associate Editor: V. T. Turitto.
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Geddes, D. M., and Cargill , R. S. (January 11, 2001). "An in Vitro Model of Neural Trauma: Device Characterization and Calcium Response to Mechanical Stretch ." ASME. J Biomech Eng. June 2001; 123(3): 247–255. https://doi.org/10.1115/1.1374201
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