Although it is well accepted that bone tissue metabolism is regulated by external mechanical loads, it remains unclear to what load-induced physical signals bone cells respond. In this study, a novel computer-controlled stretch device and parallel plate flow chamber were employed to investigate cytosolic calcium mobilization in response to a range of dynamic substrate strain levels (0.1–10 percent, 1 Hz) and oscillating fluid flow 1 Hz). In addition, we quantified the effect of dynamic substrate strain and oscillating fluid flow on the expression of mRNA for the bone matrix protein osteopontin (OPN). Our data demonstrate that continuum strain levels observed for routine physical activities (<0.5 percent) do not induce responses in osteoblastic cells in vitro. However, there was a significant increase in the number of responding cells at larger strain levels. Moreover, we found no change in osteopontin mRNA level in response to 0.5 percent strain at 1 Hz. In contrast, oscillating fluid flow predicted to occur in the lacunar–canalicular system due to routine physical activities 1 Hz) caused significant increases in both and OPN mRNA. These data suggest that, relative to fluid flow, substrate deformation may play less of a role in bone cell mechanotransduction associated with bone adaptation to routine loads. [S0148-0731(00)01204-8]
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e-mail: jyou@ortho.hmc.psu.edu
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August 2000
Technical Papers
Substrate Deformation Levels Associated With Routine Physical Activity Are Less Stimulatory to Bone Cells Relative to Loading-Induced Oscillatory Fluid Flow
J. You,
e-mail: jyou@ortho.hmc.psu.edu
J. You
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
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C. E. Yellowley,
C. E. Yellowley
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
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H. J. Donahue,
H. J. Donahue
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
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Y. Zhang,
Y. Zhang
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
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Q. Chen,
Q. Chen
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
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C. R. Jacobs
C. R. Jacobs
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
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J. You
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
e-mail: jyou@ortho.hmc.psu.edu
C. E. Yellowley
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
H. J. Donahue
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
Y. Zhang
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
Q. Chen
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
C. R. Jacobs
Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033
Contributed by the Bioengineering Division for publication in the JOURNAL OF BIOMECHANICAL ENGINEERING. Manuscript received by the Bioengineering Division July 21, 1999; revised manuscript received March 22, 2000. Associate Technical Editor: C. H. Turner.
J Biomech Eng. Aug 2000, 122(4): 387-393 (7 pages)
Published Online: March 22, 2000
Article history
Received:
July 21, 1999
Revised:
March 22, 2000
Citation
You, J., Yellowley , C. E., Donahue , H. J., Zhang , Y., Chen , Q., and Jacobs, C. R. (March 22, 2000). "Substrate Deformation Levels Associated With Routine Physical Activity Are Less Stimulatory to Bone Cells Relative to Loading-Induced Oscillatory Fluid Flow ." ASME. J Biomech Eng. August 2000; 122(4): 387–393. https://doi.org/10.1115/1.1287161
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