We have recently reported that glycation can be exploited to increase the circumferential tensile stiffness and ultimate tensile strength of media-equivalents (MEs) and increase their resistance to collagenolytic degradation, all without loss of cell viability (Girton et al., 1999). The glycated MEs were fabricated by entrapping high passage adult rat aorta SMCs in collagen gel made from pepsin-digested bovine dermal collagen, and incubated for up to 10 weeks in complete medium with 30 mM ribose added. We report here on experiments showing that ME compaction due to traction exerted by the SMCs with consequent alignment of collagen fibrils was necessary to realize the glycation-mediated stiffening and strengthening, but that synthesis of extracellular matrix constituents by these cells likely contributed little, even when 50 μg/ml ascorbate was added to the medium. These glycated MEs exhibited a compliance similar to arteries, but possessed less tensile strength and much less burst strength. MEs fabricated with low rather than high passage adult rat aorta SMCs possessed almost ten times greater tensile strength, suggesting that alternative SMCs sources and biopolymer gels may yield sufficient strength by compositional remodeling prior to implantation in addition to the structural remodeling (i.e., circumferential alignment) already obtained. [S0148-0731(00)00203-X]
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June 2000
Technical Papers
Mechanisms of Stiffening and Strengthening in Media-Equivalents Fabricated Using Glycation
T. S. Girton,
T. S. Girton
Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132
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T. R. Oegema,
T. R. Oegema
Orthopaedic Surgery, University of Minnesota, Minneapolis, MN 55455-0132
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E. D. Grassl,
E. D. Grassl
Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132
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B. C. Isenberg,
B. C. Isenberg
Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132
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R. T. Tranquillo
e-mail: tranquillo@cems.umn.edu
R. T. Tranquillo
Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132
11
Search for other works by this author on:
T. S. Girton
Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132
T. R. Oegema
Orthopaedic Surgery, University of Minnesota, Minneapolis, MN 55455-0132
E. D. Grassl
Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132
B. C. Isenberg
Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132
R. T. Tranquillo
11
Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132
e-mail: tranquillo@cems.umn.edu
Contributed by the Bioengineering Division for publication in the JOURNAL OF BIOMECHANICAL ENGINEERING. Manuscript received by the Bioengineering Division November 18, 1999; revised manuscript received February 6, 2000. Associate Technical Editor: R. Vanderby, Jr.
J Biomech Eng. Jun 2000, 122(3): 216-223 (8 pages)
Published Online: February 6, 2000
Article history
Received:
November 18, 1999
Revised:
February 6, 2000
Citation
Girton, T. S., Oegema, T. R., Grassl , E. D., Isenberg , B. C., and Tranquillo, R. T. (February 6, 2000). "Mechanisms of Stiffening and Strengthening in Media-Equivalents Fabricated Using Glycation ." ASME. J Biomech Eng. June 2000; 122(3): 216–223. https://doi.org/10.1115/1.429652
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