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ASTM Selected Technical Papers
Tissue Engineered Medical Products (TEMPs)
By
E Schutte
E Schutte
editor
Search for other works by this author on:
GL Picciolo
GL Picciolo
editor
Search for other works by this author on:
D Kaplan
D Kaplan
editor
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ISBN-10:
0-8031-3471-1
ISBN:
978-0-8031-3471-3
No. of Pages:
272
Publisher:
ASTM International
Publication date:
2004

Cells are used in bone tissue engineering applications to facilitate new bone formation in implants. Enzymatic digestion and marrow removal by either centrifugal force or syringe are three methods used to isolate the cells for culture, but each technique has benefits and drawbacks. This comparative study evaluated the effects of the three cell isolation techniques on the attachment, proliferation, and mineralization of rat bone cells. Cells were isolated, seeded, and cultured following standard protocols for each isolation method. Quantitative assays to determine metabolic activity, lactic acid production, glucose consumption, and amounts of intracellular protein, alkaline phosphatase activity, and extracellular calcium were performed. In addition, cell morphology and viability were examined qualitatively. The results indicate that the cell isolation method affects the attachment, proliferation, and type of tissue formed by cells cultured under identical conditions.

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and
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14.
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Centrifugal Isolation of Bone Marrow from Bone: An Improved Method for the Recovery and Quantitation of Bone Marrow Osteoprogenitor Cells from Rat Tibiae and Femurae
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15.
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,”
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,
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,
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,
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, Jr.
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, Jr.
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, Vol.
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, No.
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26.
Howlett
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Mineralization in in Vitro Cultures of Rabbit Marrow Stromal Cells
,”
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, Vol.
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,
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, pp. 251–263.
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