Skip to Main Content
American Society of Mechanical Engineers: Digital Collection
Cart
header search
Search
Advanced Search
Skip Nav Destination
Environmental Toxicology and Risk Assessment: Standardization of Biomarkers for Endocrine Disruption and Environmental Assessment: 8th Volume
ASTM Selected Technical Papers
Environmental Toxicology and Risk Assessment: Standardization of Biomarkers for Endocrine Disruption and Environmental Assessment: 8th Volume
By
DS Henshel
DS Henshel
1
Indiana University School of Public and Environmental Affairs
?
Bloomington, IN Symposium chair and editor
Search for other works by this author on:
This Site
PubMed
Google Scholar
MC Harrass
MC Harrass
2
BP Amoco plc
?
Warrenville, IL Symposium cochair and editor
Search for other works by this author on:
This Site
PubMed
Google Scholar
MC Black
MC Black
3
University of Georgia
Department of Environmental Health Science
Athens, GA Symposium cochair and editor
Search for other works by this author on:
This Site
PubMed
Google Scholar
ISBN-10:
0-8031-2618-2
ISBN:
978-0-8031-2618-3
No. of Pages:
496
DOI:
https://doi.org/10.1520/STP1364-EB
Publisher:
ASTM International
Publication date:
1999
eBook Chapter

Biomarkers of Endocrine Disruption at the mRNA Level

By
ND Denslow
ND Denslow
1
Director of Protein Chemistry Research Facility
, Biotechnology Program, and Department of Biochemistry and Molecular Biology,
University of Florida
,
Gainesville, FL 32610
.
Search for other works by this author on:
This Site
PubMed
Google Scholar
,
CJ Bowman
CJ Bowman
2
Graduate Student (C.J.B.) and Postdoctoral Associate (G.R.)
,
University of Florida
,
Gainesville, FL 32610
.
Search for other works by this author on:
This Site
PubMed
Google Scholar
,
G Robinson
G Robinson
2
Graduate Student (C.J.B.) and Postdoctoral Associate (G.R.)
,
University of Florida
,
Gainesville, FL 32610
.
Search for other works by this author on:
This Site
PubMed
Google Scholar
,
HS Lee
HS Lee
3
Technical Director (R.J.F.) and Research Associate (H.S.L.)
,
Differential Display Core, Cancer Center, University of Florida
,
Gainesville, FL 32610
.
Search for other works by this author on:
This Site
PubMed
Google Scholar
,
RJ Ferguson
RJ Ferguson
3
Technical Director (R.J.F.) and Research Associate (H.S.L.)
,
Differential Display Core, Cancer Center, University of Florida
,
Gainesville, FL 32610
.
Search for other works by this author on:
This Site
PubMed
Google Scholar
,
MJ Hemmer
MJ Hemmer
4
Aquatic Biologist (M.J.H.) and Research Physiologist (L.C.F.)
, Gulf Ecology Division,
Environmental Protection Agency
,
Gulf Breeze, FL 32561
.
Search for other works by this author on:
This Site
PubMed
Google Scholar
,
LC Folmar
LC Folmar
4
Aquatic Biologist (M.J.H.) and Research Physiologist (L.C.F.)
, Gulf Ecology Division,
Environmental Protection Agency
,
Gulf Breeze, FL 32561
.
Search for other works by this author on:
This Site
PubMed
Google Scholar
Doi:
https://doi.org/10.1520/STP15796S
Page Count:
12
  • Published:
    1999

A large number of estrogen-mimicking, anthropogenic chemicals capable of disrupting normal reproductive function have been identified. The ubiquitous distribution of these compounds, many as components of complex industrial or municipal waste, has spurred an effort to develop methods to screen for chemicals which disrupt normal endocrine regulation of reproduction. We have developed assays that both allow exposure of animals in vivo and measure the response at the level of gene activation. We have developed a probe for measuring the induction of vitellogenin mRNA by Northern Blot in livers of sheepshead minnows treated with 17-β-estradiol. We have also developed a strategy for using Differential Display Polymerase Chain Reaction for determining gene induction profiles following exposure to estradiol. These methods should be adaptable to a variety of structurally diverse estrogen mimics.

Keywords:
vitellogenin, estrogen receptor, estradiol, endocrine disrupters, mRNA, differential display PCR

References

1.
Arcand-Hoy
,
L.D.
, and
Benson
,
W.H.
,
1998
, “
Fish Reproduction: An Ecologically Relevant Indicator of Endocrine Disruption
,”
Environmental Toxicology and Chemistry
, Vol.
17
, pp. 49–57.
2.
Bergeron
,
J.M.
,
Crews
,
D.
, and
McLachlan
,
J.A.
,
1994
, “
PCBs as Environmental Estrogens: Turtle Sex Determination as a Biomarker of Environmental Contamination
,”
Environmental Health Perspectives
 0091-6765, Vol.
102
, pp. 780–781.
3.
Chomczynski
,
P.
, and
Sacchi
,
N.
,
1987
, “
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction
,”
Analytical Biochemistry
, Vol.
162
, pp. 56–159.
4.
Denslow
,
N.D.
,
Chow
,
M.M.
,
Folmar
,
L.C.
,
Bonomelli
,
S.
,
Heppell
,
S.A.
 and
Sullivan
,
C.V.
,
1996
, “
Development of Antibodies to Teleost Vitellogenins. Potential Biomarkers for Environmental Estrogens
,” In
Environmental Toxicology and Risk Assessment
, Vol
5
, STP 1306,
Bengston
 
D.A.
 and
Henshel
 
D.S.
, , Eds.,
American Society for Testing and Materials
,
Philadelphia, PA
, pp. 23–36.
Google Scholar
Crossref
Search ADS
 
5.
Desbrow
,
C.
,
Routledge
,
E.
,
Sheehan
,
D.
,
Waldlock
,
M.
, and
Sumpter
,
J.
,
1996
, “
The Identification and Assessment of Oestrogenic Substances in Sewage Treatment Works Effluents
,” Report P2-490/7,
United Kingdom Environment Agency, MAFF Fisheries
, Almondsbury. Bristol, UK, pp. 1–74.
6.
Donohoe
,
R.M.
, and
Curtis
,
L.R.
,
1996
, “
Estrogenic Activity of Chlordecone, o,p'-DDT, and o,p'-DDE in Juvenile Rainbow Trout: Induction of Vitellogenesis and Interaction with Hepatic Estrogen Binding Sites
,”
Aquatic Toxicology
, Vol.
36
, pp. 31–52.
7.
Feldman
,
D.
, and
Krishnan
,
A.
,
1995
, “
Estrogens in Unexpected Places: Possible Implications for Researchers and Consumers
,”
Environmental Health Perspectives
 0091-6765, Vol.
103
, pp. 129–133.
8.
Folmar
,
L.C.
,
Denslow
,
N.D.
,
Rao
,
V.
,
Chow
,
M.
,
Crain
,
D.A.
,
Enblom
,
H.
,
Marcino
,
J.
, and
Guillette
 
L.H.
 Jr.,
1996
, “
Vitellogenin Induction and Reduced Serum Testosterone Concentrations in Feral Male Carp (Cyprinus carpio) Captured Near a Major Metropolitan Sewage Treatment Plant
,”
Environmental Health Perspectives
 0091-6765, Vol.
104
, pp. 1096–1101.
9.
Flouriot
,
G.
,
Pakdel
,
F.
, and
Valotaire
,
Y.
,
1996
, “
Transcriptional and Post-transcriptional Regulation of Rainbow Trout Estrogen Receptor and Vitellogenin Gene Expression
,”
Molecular and Cellular Endocrinology
, Vol.
124
, pp. 173–183.
10.
Fry
,
D.M.
,
1995
, “
Reproductive Effects in Birds Exposed to Pesticides and Industrial Chemicals
,”
Environmental Health Perspectives
 0091-6765, Vol.
103
, pp. 165–171.
11.
Gaido
,
K.W.
,
Leonard
,
L.S.
,
Lovell
,
S.
,
Gould
,
J.C.
,
Babai
,
D.
,
Portier
,
C.J.
, and
McDonnell
,
D.P.
,
1997
, “
Evaluation of Chemicals with Endocrine Modulating Activity in a Yeast-Based Steroid Hormone Receptor Gene Transcription Assay
,”
Toxicology and Applied Pharmacology
, Vol.
143
, pp. 205–212.
12.
Gimeno
,
S.
,
Komen
,
H.
,
Venerbosch
,
P.W.M.
 and
Bowmer
,
T.
,
1997
, “
Disruption of Sexual Differentiation in Genetic Male Common Carp (Cyprinus carpio) Exposed to an Alkylphenol During Different Life Stages
,”
Environmental Science and Technology
 0013-936X, Vol.
31
, pp. 2884–2890.
13.
Harris
,
C.A.
,
Henttu
,
P.
,
Parker
,
MG.
, and
Sumpter
,
J.P.
,
1997
, “
The Estrogenic Activity of Phthalate Esters In Vitro
,”
Environmental Health Perspectives
 0091-6765, Vol.
105
, pp. 802–811.
14.
Heppell
,
S.A.
,
Denslow
,
N.D.
,
Folmar
,
L.C.
, and
Sullivan
,
C.V.
,
1996
, “
Universal Assay of Vitellogenin as a Biomarker for Environmental Estrogens
,”
Environmental Health Perspective
 0091-6765, Vol.
103
(
7
), pp. 9–15.
15.
Ignar-Trowbridge
,
D.M.
,
Pimentel
,
M.
,
Teng
,
C.T.
,
Korach
,
K.S.
, and
McLachlan
,
J.A.
,
1995
, “
Cross Talk Between Peptide Growth Factors and Estrogen Receptor Signaling Systems
,”
Environmental Health Perspectives
 0091-6765, Vol.
103
, pp. 35–38.
16.
Jobling
,
S.
,
Reynolds
,
T.
,
White
,
R.
,
Parker
,
M.G.
, and
Sumpter
,
J.P.
,
1995
, “
A Variety of Environmentally Persistent Chemicals, Including Some Phthalate Plasticizers are Weakly Estrogenic
,”
Environmental Health Perspectives
 0091-6765, Vol
103
, pp. 582–587.
17.
Jobling
,
S.
,
Sheahan
,
D.
,
Osborne
,
J.A.
,
Matthiessen
,
P.
, and
Sumpter
,
J.P.
,
1996
, “
Inhibition of Testicular Growth in Rainbow Trout (Oncorhynchus mykiss) Exposed to Estrogenic Alkylphenolic Chemicals
,”
Environmental Toxicology and Chemistry
, Vol
15
, pp. 194–202.
18.
Kelce
,
W.R.
,
Stone
,
C.R.
,
Laws
,
S.C.
,
Gray
,
L.E.
,
Kemppainen
,
J.A.
,and
Wilson
,
E.M.
,
1995
, “
Persistent DDT Metabolite p,p'-DDE is a Potent Androgen Receptor Antagonist
,”
Nature
 0028-0836, Vol.
375
, pp. 581–585.
19.
Klotz
,
D.M.
,
Beckman
,
B.S.
,
Hill
,
S.M.
,
McLachlan
,
J.A.
,
Walters
,
M.R.
, and
Arnold
,
S.F.
,
1996
, “
Identification of Environmental Chemicals with Estrogenic Activity Using a Combination of In Vitro Assays
,”
Environmental Health Perspectives
 0091-6765, Vol.
104
, pp. 1084–1089.
20.
Larsson
,
D.G. J.
,
Hyllner
,
S.J.
, and
Haux
,
C.
,
1994
, “
Induction of Vitelline Envelope Proteins by Estradiol-17B in 10 Teleost Species
,”
General and Comparative Endocrinology
, Vol.
96
, pp. 445–450.
21.
Liang
,
P.
,
Zhu
,
W.
,
Zhang
,
X.
,
Guo
,
Z.
,
O'Connell
,
R. P.
,
Averboukh
,
L.
,
Wang
,
F.
, and
Pardee
 
A. B.
,
1994
, “
Differential Display using One-base Anchored Oligo-dT Primers
,”
Nucleic Acids Research
, Vol.
22
, pp. 5763–5764.
22.
Mader
,
S.
,
Chambon
,
P.
, and
White
 
J.H.
,
1993
, “
Defining a Minimal Estrogen Receptor DNA Binding Domain
,”
Nucleic Acids Research
, Vol.
21
, pp. 1125–1132.
23.
Meek
,
M.D.
,
Clemons
,
J.H.
,
Wu
,
Z.F.
, and
Zacharewski
,
T.R.
,
1997
, “
Assessment of the Alleged Estrogen-receptor Mediated Activity of Eight Commercial Phthalate Esters
,”
Fundamental Applications in Toxicology
, Vol.
36
, pp. 295.
24.
Murata
,
K.
,
Sugiyama
,
H.
,
Yasumasu
,
S.
,
Iuchi
,
I.
,
Yasumasu
,
I.
, and
Yamagami
,
K.
,
1997
, “
Cloning of cDNA and Estrogen-induced Hepatic Gene Expression for Choriogenin H, a Precursor Protein of the Fish Egg Envelope (Chorion)
,”
Proceedings of the National Academy of Science, USA
, Vol.
94
, pp. 2050–2055.
25.
Nimrod
,
A.C.
, and
Benson
,
W.H.
,
1998
, “
Assessment of Estrogenic Activity in Fish
,” In
Chemically-Induced Alteration in the Functional Development and Reproduction of Fishes
,
Rolland
 
R.
,
Gilbertson
M
, and
Peterson
 
R.E.
 Eds.,
SETAC Press
,
Pensacola, FL
, pp. 87–100.
26.
O'Malley
,
B.W.
,
Schrader
,
W.T.
,
Mani
,
S
,
Smith
,
C.
,
Weigel
,
N.L.
,
Conneely
 
O.M.
, and
Clark
,
J.H.
,
1995
, “
An Alternative Ligand-independent Pathway for Activation of Steroid Receptors
,”
Recent Progress in Hormone Research
, Vol.
50
, pp. 333–347.
27.
Robinson
,
G.
,
Denslow
 
N.D.
,
Hemmer
 
M
. ,
Webb
 
R.
 and
Folmar
 
L.
,
1997
, “
Development of mRNA Probes for the Estrogen Receptor and Vitellogenin in the Sheepshead Minnow Model
,” Abstract to the Society of Environmental Toxicology and Chemistry, 18th Annual Meeting,
San Francisco, CA
.
28.
Routledge
,
E.J.
, and
Sumpter
,
J.P.
,
1996
, “
Estrogenic Activity of Surfactants and Some of Their Degradation Products Assessed using a Recombinant Yeast Screen
,”
Environmental Toxicology and Chemistry
, Vol.
15
, pp. 218–248.
29.
Segnitz
,
B.
, and
Gehring
,
U.
,
1995
, “
Subunit Structure of the Nonactivated Human Estrogen Receptor
,”
Proceedings of the National Academy of Sciences, USA
, Vol.
92
, pp. 2179–2183.
30.
Smith
,
D.F.
, and
Toft
,
D.O.
,
1993
, “
Steroid Receptors and their Associated Proteins
,”
Molecular Endocrinology
, Vol.
7
, pp. 4–11.
31.
Specker
,
J.L.
, and
Sullivan
,
C.V.
,
1994
“
Vitellogenesis in Fishes; Status and Perspectives
,” in
Perspectives in Comparative Endocrinology
,
Davey
 
K.G.
,
Peter
 
R.E.
 and
Tobe
 
S.S.
, Eds.,
National Research Council of Canada
, pp. 304–315.
32.
Stein
,
B.
, and
Yang
,
M.X.
,
1995
, “
Repression of the Interleukin-6-Promoter by Estrogen Receptor is Mediated by NF-kappa B and C/EBP Beta
,”
Molecular Cell Biology
, Vol.
15
, pp 4971–4979.
33.
Sumpter
,
J.P.
, and
Jobling
,
S.
,
1995
, “
Vitellogenises as a Biomarker of Estrogenic Contamination of the Aquatic Environment
,”
Environmental Health Perspectives
 0091-6765, Vol.
103
, pp. 173–178.
34.
Thomas
,
P.
, and
Smith
 
J.
,
1993
, “
Binding of Xenobiotics to the Estrogen Receptor of Spotted Seatrout: A Screening Assay for Potential Estrogenic Effects
,”
Marine Environmental Research
, Vol.
35
, pp. 147–151.
35.
Truss
,
M.
, and
Beato
 
M.
,
1993
, “
Steroid Hormone Receptors: Interaction with Deoxyribonucleic Acid and Transcription Factors
,”
Endocrinology Reviews
Vol.
14
, pp. 459–479.
36.
vom Saal
,
F.S.
,
1995
, “
Environmental Estrogenic Chemicals: Their Impact on Embryonic Development
,”
Human Ecological Risk Assessment
, Vol.
1
, pp. 3–16.
37.
Walsh
,
G.E.
, and
Banner
,
L.H.
,
1980
, “
Toxicity of Textile Mill Effluents to Freshwater and Estuarine Algae, Crustaceans and Fishes
,”
Environmental Pollution
, Vol.
21
, pp. 169–179.
38.
Zacharewski
,
T.R.
,
Berhnae
,
K.
,
Gillesby
,
B.E.
, and
Burnison
,
B.K.
,
1995
, “
Detection of Estrogen- and Dioxin-like Activity in Pulp and Paper Mill Black Liquor and Effluent Using in Vitro Recombinant Receptor/Reporter Gene Assays
,”
Environmental Science and Technology
 0013-936X, Vol.
29
, pp. 4140–4146.
39.
Zacharewski
,
T.R.
,
1997
, “
In Vitro Bioassays for Assessing Estrogenic Substances
,”
Environmental Science and Technology
 0013-936X, Vol.
31
, pp. 613–623.
This content is only available via PDF.
You do not currently have access to this chapter.

ASME Journals

ASME Conference Proceedings

ASME eBooks

Resources

Opportunities

  • Crossref Logo
  • Crossref Open Funder Registry Logo
  • Crossref Open Cited-By Logo
  • Crossref Crossmark Logo
  • Crossref Similarity Check Logo
  • Chorus Logo
  • Counter Logo
  • Cope Logo
  • STM Logo
Copyright © 2024 The American Society of Mechanical Engineers
Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.
Accept