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ASTM Selected Technical Papers
Environmental Toxicology and Risk Assessment: Modeling and Risk Assessment Sixth Volume
By
FJ Dwyer
FJ Dwyer
1
Symposium chairman and co-editor
;
Midwest Science Center
USGS-Biological Resources Division,
Columbia, MO
.
Search for other works by this author on:
TR Doane, PhD
TR Doane, PhD
2
Symposium co-chairman and co-editor
;
Battelle—San Antonio Operations
,
San Antonio, TX
.
Search for other works by this author on:
ML Hinman, PhD
ML Hinman, PhD
3
Symposium co-chairman and co-editor
;
Exxon Biomedical Sciences, Inc.
?
Millstone, NJ
.
Search for other works by this author on:
ISBN-10:
0-8031-2474-0
ISBN:
978-0-8031-2474-5
No. of Pages:
572
Publisher:
ASTM International
Publication date:
1997

Sediment toxicity assessments must address bioaccumulation potential of bound contaminants. This potential has been associated with the desorption of chemicals including many mutagens and carcinogens from sediment into interstitial waters. Environmental impacts and health hazards of sediment-bound normal, alkylated and heterocyclic aromatic hydrocarbons are functions of their entry into food webs, the activation of promutagens, and bioaccumulation processes. Activation phenomena occurring in benthic organisms may generate genotoxic agents not extractable with non-polar solvents used for chemical analysis.

Co-solvent theory was applied to prepare aqueous-based extracts from petroleum contaminated sediments from Pass Fourchon, LA. Co-solvent solubilization of contaminants is thermodynamically related to partition coefficients which govern bioavailability to benthic organisms. Sediments were extracted into acetic acid co-solvent mixtures (0, 10, 20, and 50 %, in seawater) and mutagens mobilized into the extracts were detected by the Salmonella typhimurium mutagenicity assay with and without activation. Direct-acting mutagens were mobilized at all co-solvent concentrations, causing reversions in strain TA102, but not in TA97a, TA98, or TA100. Promutagens were detectable in all extracts after activation with Arochlor-induced rat liver S9. Increasing co-solvent strength seemed to suppress the extraction of water soluble mutagens but enhanced the extraction of lipophilic mutagens. Differing sensitivities were noted among the tester strains, with TA102 being most sensitive and TA98 showing no response to the mutagens. Mutagenicity varied among co-solvent concentrations, perhaps reflecting the mobilization of different arrays of compounds. Chemical analysis of co-solvent extracts by GC/MS yielded identifications of possible mutagenic constituents.

1.
Banwart
,
W. L.
,
Hassett
,
J. J.
,
Wood
,
S. G.
, and
Means
,
J. C.
,
1982
, “
Sorption of Nitrogen Heterocyclic Compounds on Sediments and Soils
”,
Soil Sci.
 0038-075X, Vol.
133
, pp. 42–47.
2.
Capuzzo
,
J. M.
,
1987
, “
Biological effects of Petroleum Hydrocarbons: Assessment from Experimental Results
”, pp. 343–410 in
Boesch
,
D. F.
, and
Rabalais
,
N. N.
(eds), Long-Term Environmental Effects of Offshore Oil and Gas Development,
Elsevier Applied Science
,
New York, NY
3.
Daniels
,
C. B.
,
Baksi
,
S. M.
,
Uhler
,
A. D.
, and
Means
,
J. C.
,
1985
, “
Effects of Chlorination on Levels of Mutagens in Contaminated Estuarine Sediments
”, pp. 1411–1424 in
Jolley
,
R. L.
,
Bull
,
R. J.
,
Davis
,
W. P.
,
Katz
,
S.
,
Roberts
,
M. H.
, Jr.
, and
Jacobs
,
V. A.
(eds), Water Chlorination: Chemistry, Environmental Impact and Health Effects, Vol.
5
,
Lewis Publishers, Inc.
,
Chelsea, MI
4.
Dexter
,
R. N.
,
Pavlou
,
S. P.
, and
Kocan
,
R. M.
,
1979
, “
Mutagenicity of Puget Sound Sediment Extract: Feasibility Study
”, Report PB 80-152770
National Oceanographic and Atmospheric Administration
, Boulder, CO
5.
Farrington
,
J. W.
,
Goldberg
,
E. D.
,
Risebrough
,
R. W.
,
Martin
,
J. H.
, and
Bowden
,
V.T.
,
1983
, “
U.S. Mussel Watch 1967–1978: An Overview of the Trace Metal, DDE, PCB, Hydrocarbon, and Artificial Radionuclide Data
”.
Environ. Sci. Technol.
 0013-936X, Vol.
17
, pp. 490–496
6.
Foster
,
G. D.
,
Baksi
,
S. M.
, and
Means
,
J. C.
,
1987
, “
Bioaccumulation of a Mixture of Sediment-Associated Organic Contaminants by the Baltic Clam (Macoma Balthica) and Soft Shell Clam (Mya arenaria)
”,
J. Envir. Toxicol. Chem.
, Vol.
6
, pp. 969–976
7.
Hassett
,
J. J.
,
Means
,
J. C.
,
Banwart
,
W. L.
,
Wood
,
S. G.
,
Ali
,
S.
, and
Khan
,
A.
,
1980
, “
Sorption of Dibenzothiophene by Soils and Sediments
”,
J. Environ. Qual.
, Vol.
9
, pp. 184–186
8.
Maron
,
D. M.
and
Ames
,
B. N.
,
1983
, “
Revised Methods for the Salmonella Mutagenicity Test
”,
Mutat.Res.
, Vol.
113
, pp. 173–215
9.
McElroy
,
A. E.
, and
Means
,
J. C.
,
1988
, “
Factors Influencing the Bioavailability of Hexachlorobiphenyl to Benthic Organisms
”, Aquatic Toxicology and Hazard Assessment, Vol.
10
, ASTM STP 971,
ASTM
, pp 149–158
10.
McMillin
,
D. J.
, and
Means
,
J. C.
,
11
1996
, “
Spatial and Temporal Trends of Pesticide Residues in Water and Particulates in the Mississippi River Plume and Northwestern Gulf of Mexico
”,
J. Chrom.
, in press
11.
Means
,
J. C.
,
Wood
,
S. G.
,
Hassett
,
J. J.
, and
Banwart
,
W. L.
,
1982
, “
Sorption Of Amino-and Carboxy-Substituted Polynuclear Aromatic Hydrocarbons on Sediments and Soils
”,
Envir. Sci. Tech.
 0013-936X, Vol.
16
, pp. 93–98
12.
Means
,
J. C.
,
Wood
,
S. G.
,
Hassett
,
J. J.
, and
Banwart
,
W. L.
,
1980
, “
Sorption Properties of Polynuclear Aromatic Hydrocarbons by Sediments and Soils
”,
Envir. Sci.Tech.
 0013-936X, Vol.
14
, pp. 1524–1528
13.
Means
,
J. C.
, and
Wijayaratne
,
R.
,
1982
, “
Role of Natural Colloids in Transport of Hydrophobic Pollutants
”,
Science
 0036-8075, Vol.
215
, pp. 968–970
14.
Means
,
J. C.
, and
McMillin
,
D. J.
,
1993
, “
Fate and Transport of Particle-Reactive Normal, Alkylated and Heterocyclic Aromatic Hydrocarbons in a Sediment-Water-Colloid System.
” Final Report USMMS OCS Study MMS-93-0018, p. 153
15.
Means
,
J. C.
,
Zoeller
,
A. L.
, and
Ma
,
J.-W.
,
1988
, “
Investigation of the Use of Co-solvents for Enhanced Hazardous Waste Recovery from Contaminated Soils
”, Final Report to Louisiana Department of Environmental Quality, Baton Rouge, LA
16.
Neff
,
J. M.
,
Cox
,
B. A.
,
Dixit
,
D.
, and
Anderson
,
J. W.
,
1976
, “
Accumulation and Release of Petroleum-Derived Aromatic Hydrocarbons by Four Marine Animals
”,
Mar.Biol.
, Vol.
38
, pp. 279–289
17.
Osborne
,
L. L.
,
Davies
,
R. W.
,
Dixon
,
K. R.
, and
Moore
,
R. L.
,
1982
, “
Mutagenicity of Fish and Sediment in Sheep River, Alberta
”,
Water Res.
 0043-1354, Vol.
16
, pp. 899–902
18.
NOAA
,
1988
,
National Status and Trends Program Progress Report: A Summary of Selected Data on Chemical Contaminants in Tissues Collected During 1984, 1985, and 1986
,
National Oceanic and Atmospheric Administration
, Rockville, MD
19.
Rabalais
,
N. N.
,
Means
,
J. C.
, and
Boesch
,
D. F.
,
1991
, “
Fate and Effects of Produced Water Discharges in Coastal Environments
”, Proc. 1st. Intern. Symp. Oil and Gas Waste Management,
New Orleans
, pp. 503–514
20.
Rao
,
P. S. C.
,
Hornsby
,
A. G.
,
Kilcrease
,
D. P.
, and
Nkedi-Kizza
,
P. J.
,
1985
, “
Sorption and Transport of Hydrophobic Organic Molecules in Aqueous and Mixed Solvent Systems
”,
J. Envir. Oual.
, Vol.
14
, pp. 376–382
21.
Sigleo
,
A. C.
, and
Means
,
J. C.
,
1990
, “
Organic and Inorganic Components of Estuarine Colloids: Implications for Transport of Pollutants
Rev. Envir. Toxicol. Contam.
, Vol.
112
, pp. 123–147
22.
Suzuki
,
J.
,
Sadamasu
,
T.
, and
Suzuki
,
S.
,
1982
, “
Mutagenic Activity of Organic Matter in Urban Sediment
”,
Environ. Pollut. Ser.
, Vol.
A29
, pp. 91–99
23.
Wijayaratne
,
R. D.
, and
Means
,
J. C.
,
1984
, “
Sorption of Polycyclic Aromatic Hydrocarbons (PAHs) by Natural Colloids
”,
Marine Environ. Res.
, Vol.
11
, pp. 77–89
24.
Winston
,
G.W.
and
Means
,
J.C.
,
1995
, “
Bioavailability and Genotoxicity of Produced Water Discharges Associated with Offshore Production Operations
”, Final Report USMMSPCS Study MMS-95-0020, p 112.
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