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ASTM Selected Technical Papers
Environmental Toxicology and Risk Assessment: Modeling and Risk Assessment Sixth Volume
By
FJ Dwyer
FJ Dwyer
1
Symposium chairman and co-editor
;
Midwest Science Center
USGS-Biological Resources Division,
Columbia, MO
.
Search for other works by this author on:
TR Doane, PhD
TR Doane, PhD
2
Symposium co-chairman and co-editor
;
Battelle—San Antonio Operations
,
San Antonio, TX
.
Search for other works by this author on:
ML Hinman, PhD
ML Hinman, PhD
3
Symposium co-chairman and co-editor
;
Exxon Biomedical Sciences, Inc.
?
Millstone, NJ
.
Search for other works by this author on:
ISBN-10:
0-8031-2474-0
ISBN:
978-0-8031-2474-5
No. of Pages:
572
Publisher:
ASTM International
Publication date:
1997

Few non-mammalian systems have been used as models for assessing the developmental toxicity of environmental contaminants although the U. S. Environmental Protection Agency recognizes mammalian in vitro systems as appropriate developmental toxicity screens. The chick embryo micromass cell culture system was tested for its predictability to screen developmental toxicants to the skeletal system. Four toxicants with known toxicities in the rodent limb bud cell culture system (arsenate, aspirin, caffeine and methylmercury) were chosen for testing in the chick culture system. Cartilage-specific products, proteoglycans, were used to determine differentiation of the cells in culture by staining with Alcian Green then measured using a spectrophotometric method. Proliferation was determined by staining with Crystal Violet. Dose and temporal response experiments were conducted to determine the most sensitive dose and time of exposure to cells in culture. Overall, the micromass cultures of the chick limb buds responded in a manner similar to those seen in the rodent culture.

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.”
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