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ASTM Selected Technical Papers
Native Aquatic Bacteria: Enumeration, Activity, and Ecology
By
JW Costerton
JW Costerton
1
University of Calgary
,
Calgary, Alberta,
Canada
;
symposium cochairman and coeditor
.
Search for other works by this author on:
RR Colwell
RR Colwell
2
University of Calgary
,
Calgary, Alberta,
Canada
;
symposium cochairman and coeditor
.
Search for other works by this author on:
ISBN-10:
0-8031-0526-6
ISBN:
978-0-8031-0526-3
No. of Pages:
222
Publisher:
ASTM International
Publication date:
1979

The biomass of bacteria in various waters can be estimated by measuring the amount of cellular bound lipopolysaccharide (LPS). LPS, which is present in the cell walls of gram-negative bacteria, including cyanobacteria, can be quantitated by using an extract of Limulus amebocytes. In one special environment, an oceanic water column below the euphotic zone, the results of the LPS technique agreed very well with those of the direct count and adenosine triphosphate techniques for measuring bacterial biomass.

1.
Fliermans
,
C. B.
,
Cain
,
P.
, and
Schmidt
,
E. L.
,
Archives of Hydrobiology
, Vol.
76
,
1975
, pp. 248–255.
2.
Francisco
,
D. E.
,
Mah
,
R. A.
, and
Rabin
,
A. C.
,
Transactions of the American Microscopial Society
, Vol.
92
,
1973
, pp. 416–421.
3.
Jones
,
J. G.
and
Simon
,
B. M.
,
Journal of Applied Bacteriology
 0021-8847, Vol.
39
,
1975
, pp. 1–13.
4.
Zimmerman
,
R.
and
Meyer-Reil
,
L.
,
Kieler Meeresforschungen
, Vol.
30
,
1974
, pp. 24–27.
5.
Hobbie
,
J. E.
,
Daley
,
R. J.
, and
Jasper
,
S.
,
Applied Environmental Microbiology
 0099-2240, Vol.
33
,
1977
, pp. 1225–1228.
6.
Watson
,
S. W.
,
Novitsky
,
T. J.
,
Quinby
,
H. L.
, and
Valois
,
F. W.
,
Applied Environmental Microbiology
 0099-2240, Vol.
33
,
1977
, pp. 940–946.
7.
Levin
,
J.
and
Bang
,
F. B.
,
Bulletin of the Johns Hopkins Hospital
, Vol.
115
,
1964
, pp. 337.
8.
Holm-Hansen
,
O.
and
Booth
,
C. R.
,
Limnology and Oceanography
 0024-3590, Vol.
11
,
1966
, pp. 510–519.
9.
Novitsky
,
T. J.
,
Passman
,
F. J.
, and
Watson
,
S. W.
, “
Relationship Between Bacterial Direct Count and Lipopolysaccharide (LPS) in Marine Sediments
,” presented at the 78th Annual Meeting,
American Society for Microbiology
,
Las Vegas
,
1978
.
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