Most of previous researches of inlet turbulence effects on blade tip have been carried out for low speed situations. Recent work has indicated that for a transonic turbine tip, turbulent diffusion tends to have distinctively different impact on tip heat transfer than for its subsonic counterpart. It is hence of interest to examine how inlet turbulence flow conditioning would affect heat transfer characteristics for a transonic tip. This present work is aimed to identify and understand the effects of both inlet freestream turbulence and end-wall boundary layer on a transonic turbine blade tip aero-thermal performance. Spatially-resolved heat transfer data are obtained at aerodynamic conditions representative of a high-pressure turbine, using the transient infrared thermography technique with the Oxford High-Speed Linear Cascade research facility. With and without turbulence grids, the turbulence levels achieved are 7–9% and 1% respectively. On the blade tip surface, no apparent change in heat transfer was observed with high and low turbulence intensity levels investigated. On the blade suction surface, however, substantially different local heat transfer for the suction side near tip surface have been observed, indicating a strong local dependence of the local vortical flow on the freestream turbulence. These experimentally observed trends have also been confirmed by CFD predictions using Rolls-Royce HYDRA. Further CFD analysis suggests that the level of inflow turbulence alters the balance between the passage vortex associated secondary flow and the OverTL flow. Consequently, enhanced inertia of near wall fluid at a higher inflow turbulence weakens the cross-passage flow. As such, the weaker passage vortex leads the tip leakage vortex to move further into the mid passage, with the less spanwise coverage on the suction surface, as consistently indicated by the heat transfer signature. Different inlet end-wall boundary layer profiles are employed in the HYDRA numerical study. All CFD results indicate the inlet boundary layer thickness has little impact on the heat transfer over the tip surface as well as the pressure side near-tip surface. However, noticeable changes in heat transfer are observed for the suction side near-tip surface. Similar to the freestream turbulence effect, such changes are attributed to the interaction between the passage vortex and the OTL flow.