Conducting polymers possess similarity in ion transport function to cell membranes and perform electro-chemo-mechanical energy conversion. In an in vitro setup, protein-reconstituted bilayer lipid membranes (bioderived membranes)perform similar energy conversion and behave like cell membranes. Inspired by the similarity in ionic function between a conducting polymer membrane and cell membrane, this article presents a thin-film laminated membrane in which alamethicin-reconstituted lipid bilayer membrane is supported on a polypyrrole membrane. Owing to the synthetic and bioderived nature of the components of the membrane, we refer to the laminated membrane as a hybrid bioderived membrane. In this article, we describe the fabrication steps and electrochemical characterization of the hybrid membrane. The fabrication steps include electropolymerization of pyrrole and vesicle fusion to result in a hybrid membrane; and the characterization involves electrical impedance spectroscopy, chronoamperometry and cyclic voltammetry. The resistance and capacitance of BLM have the magnitude of 4.6×109Ω-cm2 and 1.6×10−8 F/cm2.The conductance of alamethicin has the magnitude of 6.4×10−8 S/cm2. The change in ionic conductance of the bioderived membrane is due to the electrical field applied across alamethicin, a voltage-gated protein and produces a measurable change in the ionic concentration of the conducting polymer substrate.
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ASME 2011 Conference on Smart Materials, Adaptive Structures and Intelligent Systems
September 18–21, 2011
Scottsdale, Arizona, USA
Conference Sponsors:
- Aerospace Division
ISBN:
978-0-7918-5472-3
PROCEEDINGS PAPER
Electrochemical Analysis of Alamethicin Reconstituted Planar Bilayer Lipid Membranes Supported on Polypyrrole Membranes
Hao Zhang,
Hao Zhang
Virginia Commonwealth University, Richmond, VA
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Vishnu Baba Sundaresan,
Vishnu Baba Sundaresan
Virginia Commonwealth University, Richmond, VA
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Sergio Salinas,
Sergio Salinas
Virginia Commonwealth University, Richmond, VA
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Robert Northcutt
Robert Northcutt
Virginia Commonwealth University, Richmond, VA
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Hao Zhang
Virginia Commonwealth University, Richmond, VA
Vishnu Baba Sundaresan
Virginia Commonwealth University, Richmond, VA
Sergio Salinas
Virginia Commonwealth University, Richmond, VA
Robert Northcutt
Virginia Commonwealth University, Richmond, VA
Paper No:
SMASIS2011-5038, pp. 629-635; 7 pages
Published Online:
February 7, 2012
Citation
Zhang, H, Sundaresan, VB, Salinas, S, & Northcutt, R. "Electrochemical Analysis of Alamethicin Reconstituted Planar Bilayer Lipid Membranes Supported on Polypyrrole Membranes." Proceedings of the ASME 2011 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. ASME 2011 Conference on Smart Materials, Adaptive Structures and Intelligent Systems, Volume 2. Scottsdale, Arizona, USA. September 18–21, 2011. pp. 629-635. ASME. https://doi.org/10.1115/SMASIS2011-5038
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