Applying feedback control strategies to biological materials establishes a new paradigm for creating controlled biomolecular systems. Specifically, current tracking and feedback voltage amplification are demonstrated separately on bilayer lipid membranes (BLMs) formed via the droplet-interface bilayer (DIB) method. Ion channel induced degradation of the bilayer is studied in order to provide a convenient method for causing changes to the bilayer which can be monitored using proportional-integral (PI) feedback voltage control. Alpha-hemolysin (αHL) from Staphylococcus aureus was shown to cause large scale reductions (+90%) to the resistance of the lipid bilayers formed at the interface of connected water droplets within 90 minutes of bilayer formation. Feedback integral current control was demonstrated on pure 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) DIBs not containing αHL and provided accurate current tracking of a 100pA desired current signal driven at a rate of 10mHz and less. Voltage amplification monitoring was achieved on DPhPC DIBs containing αHL, providing a way to detect decreasing resistance and capacitance of the bilayer and nonlinear current-voltage relationship.
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Feedback Control of Biomolecular Systems Formed From Droplet-Interface Bilayers
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Sarles, SA, & Leo, DJ. "Feedback Control of Biomolecular Systems Formed From Droplet-Interface Bilayers." Proceedings of the ASME 2008 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. Smart Materials, Adaptive Structures and Intelligent Systems, Volume 2. Ellicott City, Maryland, USA. October 28–30, 2008. pp. 361-375. ASME. https://doi.org/10.1115/SMASIS2008-421
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