Collagen gel tissue-equivalents (TEs), which are simple model tissues with tunable properties, have been used to explore many properties of soft tissues, such as how structural and compositional properties affect mechanical function [1–4]. One aspect not captured in previous TE formulations is residual stress due to interactions among components, which has an important functional role in many tissues (e.g., blood vessels , ligaments , annulus fibrosus ). Since the in vivo stress state of native tissues is not easily replicated in TE fabrication, a different method for “pre-stressing” collagen networks of TEs was necessary. To this end, co-gel TEs were fabricated by adding hyaluronic acid (HA) to reconstituted Type-I collagen (Col) gels. When placed in solutions of varying osmolarity, HA-Col TEs swell as the HA binds water, which in turn will stretch (and stress) the collagen network. In this way, TEs with residual stress (i.e., pre-stressed collagen fibers) can be fabricated and evaluated in order to elucidate relationships between residual stress and functional properties. Therefore, the goals of the present study were to fabricate HA-Col TEs, make initial measurements of their swelling properties, and quantify the mechanical response and changes in microstructural organization under applied tensile load.
- Bioengineering Division
Swelling of Collagen-Hyaluronic Acid Tissue-Equivalents: An Experimental Model to Evaluate Residual Stress in Soft Tissues
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Lai, VK, Lake, SP, Kim, B, Weiss, EM, Tranquillo, RT, & Barocas, VH. "Swelling of Collagen-Hyaluronic Acid Tissue-Equivalents: An Experimental Model to Evaluate Residual Stress in Soft Tissues." Proceedings of the ASME 2013 Summer Bioengineering Conference. Volume 1B: Extremity; Fluid Mechanics; Gait; Growth, Remodeling, and Repair; Heart Valves; Injury Biomechanics; Mechanotransduction and Sub-Cellular Biophysics; MultiScale Biotransport; Muscle, Tendon and Ligament; Musculoskeletal Devices; Multiscale Mechanics; Thermal Medicine; Ocular Biomechanics; Pediatric Hemodynamics; Pericellular Phenomena; Tissue Mechanics; Biotransport Design and Devices; Spine; Stent Device Hemodynamics; Vascular Solid Mechanics; Student Paper and Design Competitions. Sunriver, Oregon, USA. June 26–29, 2013. V01BT54A002. ASME. https://doi.org/10.1115/SBC2013-14609
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