Hydrogel-based tissue engineering scaffolds can allow tissues to repair and regenerate by providing a 3D environment similar to soft tissue. Type I collagen has the ability to assemble into a fibrillar gel at physiological temperature and pH, while promoting cell adhesion and growth. Our lab has modified type I collagen by covalently adding methacrylate groups to lysine residues to create collagen methacrylamide (CMA). This biomaterial, like collagen, maintains the ability to self-assemble, and can then be photocrosslinked with long-wave UV light and a water-soluble photoinitiator, which allows extensive spatiotemporal control of mechanical and biochemical properties . In characterizing CMA and developing it for other applications, we discovered an interesting property. Unlike type I collagen hydrogels, which maintain a stable fibrillar network during cooling and freezing, CMA will spontaneously disassemble at temperatures less than 10°C. In this paper, we discuss the temperature-dependent rheological properties of CMA as well as the nature of its molecular and supramolecular structure in comparison to collagen.
- Bioengineering Division
Temperature Dependent Reversible Self Assembly of Methacrylated Collagen Gels
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Drzewiecki, K, Gaudet, I, Pike, D, Branch, J, Nanda, V, & Shreiber, D. "Temperature Dependent Reversible Self Assembly of Methacrylated Collagen Gels." Proceedings of the ASME 2013 Summer Bioengineering Conference. Volume 1B: Extremity; Fluid Mechanics; Gait; Growth, Remodeling, and Repair; Heart Valves; Injury Biomechanics; Mechanotransduction and Sub-Cellular Biophysics; MultiScale Biotransport; Muscle, Tendon and Ligament; Musculoskeletal Devices; Multiscale Mechanics; Thermal Medicine; Ocular Biomechanics; Pediatric Hemodynamics; Pericellular Phenomena; Tissue Mechanics; Biotransport Design and Devices; Spine; Stent Device Hemodynamics; Vascular Solid Mechanics; Student Paper and Design Competitions. Sunriver, Oregon, USA. June 26–29, 2013. V01BT50A005. ASME. https://doi.org/10.1115/SBC2013-14705
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