In this work, the dilution-concentration method proposed in our previous work is optimized to reduce the osmotic damage of red blood cells during the process of removing cryoprotective agents from cryopreserved blood. In the optimization, the dilution region i.e. the tubing length from the dilution point to the concentration point is extended so that the negative accumulation effect of the volume change of red blood cells can be allivated. By doing this, the volume maximum of red blood cells experienced during the removal of cryoprotective agents can be decreased and thereby the osmotic damage of red blood cells can be reduced.

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