Regarding to the advances in mechanical stimulation of cells, this study aims to address important issues in bone generation and therapy at cellular level as it relates to understanding of bone cell response to hydrostatic pressure as well as choosing a proper cell model in studies of bone cell response to mechanical stimulation. G292 human osteosarcoma cell line and human primary osteoblast cells were tested under cyclic hydrostatic pressure. Monolayer culture of cells were divided into three groups of control without loading, static with the pressure of 0.5 MPa, and dynamic with the pressure of 0.5 MPa and the frequency of 1 Hz. The cells were analyzed with measurement of alkaline phosphatase activity. Higher level of alkaline phosphatase activity in all groups of primary cell model compared with cell line model indicated more sensitivity of response of primary cells to this kind of loading.

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