Circulating white blood cells adhere to endothelium near an infection site; this occurs because infection causes ligands to be expressed on activated endothelium. Initially, a white blood cell rolls on the substrate, but eventually forms a firm adhesion, allowing it to crawl through the endothelial layer toward the infected tissue. A computational model of bond kinetics, cell deformability, and fluid dynamics was used to model the forces experienced by a cell during this process. The cell was modeled as a fluid-filled membrane; on its surface were hundreds of deformable microvilli—little fingers, ruffles in the white blood cell’s wrinkly membrane. These microvilli were deformable and their tips were decorated with PSGL-1 chemical receptors which bound to P-selectin ligands on the surface. Softer cells and cells subjected to higher fluid shear stress deformed more, and having more contact area, they formed more bonds and were able to resist more hydrodynamic load.
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ASME 2012 Summer Bioengineering Conference
June 20–23, 2012
Fajardo, Puerto Rico, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4480-9
PROCEEDINGS PAPER
Deformation of White Blood Cells Firmly Adhered to Endothelium Available to Purchase
Alex C. Szatmary,
Alex C. Szatmary
University of Maryland, Baltimore County, Baltimore, MD
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Rohan J. Banton,
Rohan J. Banton
U.S. Army Research Laboratory, Aberdeen Proving Ground, MD
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Charles D. Eggleton
Charles D. Eggleton
University of Maryland, Baltimore County, Baltimore, MD
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Alex C. Szatmary
University of Maryland, Baltimore County, Baltimore, MD
Rohan J. Banton
U.S. Army Research Laboratory, Aberdeen Proving Ground, MD
Charles D. Eggleton
University of Maryland, Baltimore County, Baltimore, MD
Paper No:
SBC2012-80894, pp. 697-698; 2 pages
Published Online:
July 19, 2013
Citation
Szatmary, AC, Banton, RJ, & Eggleton, CD. "Deformation of White Blood Cells Firmly Adhered to Endothelium." Proceedings of the ASME 2012 Summer Bioengineering Conference. ASME 2012 Summer Bioengineering Conference, Parts A and B. Fajardo, Puerto Rico, USA. June 20–23, 2012. pp. 697-698. ASME. https://doi.org/10.1115/SBC2012-80894
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